三裂叶薯NBS-LRR类抗病基因的筛选鉴定与结构分析

Screening and Identification and Structural Analysis of NBS-LRR Family Genes in Ipomoea triloba

为发掘甘薯近缘野生种三裂叶薯(Ipomoea triloba)的NBS-LRR类抗病基因,从基因数据库中对三裂叶薯基因组序列进行了筛选、鉴定和分析。结果表明,从三裂叶薯的98025个基因中,筛选到282个编码NBS-LRR类蛋白的基因,其中N型80个,NL型83个,CN型28个,CNL型57个,TN型10个,TNL型23个,RN型1个。三裂叶薯的16条染色体上均含有NBS-LRR家族基因,数量最多的染色体含有65个,最少的只有1个。三裂叶薯基因组共有55个基因簇,包含了63.5%的NBS-LRR家族基因。在NBS-LRR抗病基因家族中,CNL和TNL亚家族分别对应到7和11个保守结构域。这为三裂叶薯抗性资源的利用提供了科学参考。

In order to exploit the NBS-LRR resistance genes of Ipomoea triloba,a close wild relative of I.batatas,the genome sequence was screened,identified and analyzed by using pertinent software.The results showed that among the 98025 genes identified from I.triloba,there are 282 genes encoding NBS-LRR proteins,including 80 N type,83 NL type,28 CN type,57 CNL type,10 TN type,23 TNL and 1 RN type.NBS-LRR family genes were located in all of 16 chromosomes,each containing 1 to 65 genes.There were 55 gene clusters in genome of I.triloba,containing 63.5%of the NBS-LRR family genes.There were 7 and 11 conservative domains in CNL and TNL subfamilies,respectively.Therefore,these would provide a scientific reference for utilization of resistance resource in I.triloba.