摘要
【目的】了解洋底深部真菌适应原位环境的生长特征。【方法】在模拟洋底原位环境因子(压力除外)的培养条件(温度,pH,盐度,Fe2+,木质素和NH4+)下,比较了4株分离自洋底约2 km深处含煤沉积物的裂褶菌和2株分离自海洋及陆地生境的裂褶菌的生长速率。【结果】在本实验所设置的温度(20,30,40,45℃)、氧气(有氧和无氧)、pH(6,8,10)、盐度(淡水,原位水,人工海水)、Fe^2+(0.27,8.93,89.28μmol/L)、木质素(1,5,10 g/L)和NH4+(0.5,1.0,5.0 g/L)条件下,洋底菌株均比陆地菌株(CFCC7252)和海洋菌株(MCCC 3A00233)生长快,但不同洋底菌株之间也存在生长差异,菌株6R-2-F01和24R-3-F01在厌氧条件下的生长速率显著高于其在有氧条件下的生长速率。【结论】洋底真菌(如裂褶菌)可能拥有独特的生物学特性,以帮助其适应洋底极端环境,相关研究值得进一步探讨。
[Objective]To understand the growth characteristics of deep subseafloor fungi adapted to the in-situ environment.[Methods]The growth rates of four strains of Schizophyllum commune isolated from coal-bearing sediments about 2 km below the seafloor and two strains isolated from marine and terrestrial habitats were studied by comparative culture under different environmental factors,mimicking the in-situ conditions(except for pressure)such as temperature,pH,salinity,Fe2+,lignin,and NH4+.[Results]All the strains of subseafloor fungus grew significantly better than terrestrial(CFCC7252)and marine(MCCC 3A00233)isolated strains under the given culture conditions,including temperature(20,30,40,45℃),oxygen(aerobic and anaerobic),pH(6,8,10),salinity(freshwater,in-situ water,artificial seawater),Fe^2+(0.27,8.93,89.28μmol/L),lignin(1,5,10 g/L),and NH4+(0.5,1.0,5.0 g/L).However,there were also differences among different strains in the sedimentary environment below seafloor.The growth rate of strains 6R-2-F01 and 24R-3-F01 under anaerobic conditions was significantly higher than that of aerobic conditions.[Conclusion]Fungi from the subseafloor,such as S.commune,may have distinct biological traits that help them adapt to the extreme marine subsurface environments.
作者
M.Zain Ul Arifeen
杨心怡
李方方
薛雅蓉
公丕贤
刘常宏
M.Zain Ul Arifeen;Xinyi Yang;Fangfang Li;Yarong Xue;Pixian Gong;Changhong Liu(State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences,Nanjing University,Nanjing 210023,Jiangsu Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2020年第9期1882-1892,共11页
Acta Microbiologica Sinica
基金
国家自然科学基金(91951121,41773083,41973073)。
关键词
洋底
真菌
裂褶菌
适应性
生态系统
生物圈
subseafloor
fungi
Schizophyllum commune
adaptation
ecosystem
biosphere