洋底深部裂褶菌在不同环境条件的生长行为

Growth behaviors of deep subseafloor Schizophyllum commune in response to various environmental conditions

【目的】了解洋底深部真菌适应原位环境的生长特征。【方法】在模拟洋底原位环境因子(压力除外)的培养条件(温度,pH,盐度,Fe2+,木质素和NH4+)下,比较了4株分离自洋底约2 km深处含煤沉积物的裂褶菌和2株分离自海洋及陆地生境的裂褶菌的生长速率。【结果】在本实验所设置的温度(20,30,40,45℃)、氧气(有氧和无氧)、pH(6,8,10)、盐度(淡水,原位水,人工海水)、Fe^2+(0.27,8.93,89.28μmol/L)、木质素(1,5,10 g/L)和NH4+(0.5,1.0,5.0 g/L)条件下,洋底菌株均比陆地菌株(CFCC7252)和海洋菌株(MCCC 3A00233)生长快,但不同洋底菌株之间也存在生长差异,菌株6R-2-F01和24R-3-F01在厌氧条件下的生长速率显著高于其在有氧条件下的生长速率。【结论】洋底真菌(如裂褶菌)可能拥有独特的生物学特性,以帮助其适应洋底极端环境,相关研究值得进一步探讨。

[Objective]To understand the growth characteristics of deep subseafloor fungi adapted to the in-situ environment.[Methods]The growth rates of four strains of Schizophyllum commune isolated from coal-bearing sediments about 2 km below the seafloor and two strains isolated from marine and terrestrial habitats were studied by comparative culture under different environmental factors,mimicking the in-situ conditions(except for pressure)such as temperature,pH,salinity,Fe2+,lignin,and NH4+.[Results]All the strains of subseafloor fungus grew significantly better than terrestrial(CFCC7252)and marine(MCCC 3A00233)isolated strains under the given culture conditions,including temperature(20,30,40,45℃),oxygen(aerobic and anaerobic),pH(6,8,10),salinity(freshwater,in-situ water,artificial seawater),Fe^2+(0.27,8.93,89.28μmol/L),lignin(1,5,10 g/L),and NH4+(0.5,1.0,5.0 g/L).However,there were also differences among different strains in the sedimentary environment below seafloor.The growth rate of strains 6R-2-F01 and 24R-3-F01 under anaerobic conditions was significantly higher than that of aerobic conditions.[Conclusion]Fungi from the subseafloor,such as S.commune,may have distinct biological traits that help them adapt to the extreme marine subsurface environments.