基质Gla蛋白对骨量的调节作用及其机制

Modulatory effect of matrix Gla protein on bone mass and underlying mechanism

目的检测基质Gla蛋白(MGP)对小鼠骨量的影响,分析其作用机制。方法利用腺相关病毒干涉小鼠体内MGP基因表达,microCT扫描分析检测小鼠骨量,利用抗酒石酸酸性磷酸酶(TRAP)染色检测小鼠股骨破骨细胞的数目,利用ELISA检测骨转换标志物TRAP5b水平。结果与对照组相比,经过MGP干扰处理的小鼠骨体积分数显著降低,骨小梁数目和骨小梁厚度也显著减少,骨小梁间隔显著增高,差异均有统计学意义(均P<0.01)。小鼠股骨切片TRAP阳性破骨细胞数目增多,血清TRAP5b水平显著增加(P<0.01)。结论MGP可能通过抑制破骨细胞形成和骨吸收来维持骨量。

Objective To detect effects of matrix Gla protein(MGP)on bone mass and the underlying mechanism.Methods MGP expression was interfered with recombinant adeno-associated virus(r AAV)in mice.The bone mass was examined by microCT analysis.The formation of osteoclasts was determined by tartrate resistant acid phosphatase(TRAP)staining.The serum level of TRAP5 b was assessed by ELISA.Results Compared with the control group,the bone volume fraction of mice treated with MGP interference was significantly reduced,the number and the thickness of the trabecular bone were also significantly reduced,and the interval of the trabecular bone was significantly increased(P<0.01 for all).The number of TRAP-positive osteoclasts in mouse femoral slices increased,and the level of serum TRAP5 b increased significantly(P<0.01).Conclusion MGP may regulate bone mass by inhibiting osteoclast formation and bone resorption.