浮游态白念珠菌SC5314标准株及耐药株自噬的研究

Study on autophagy of standard strains and drug-resistant strains in Candida albicans SC5314

目的比较白念珠菌浮游状态耐药株与标准株自噬的差异,初步探讨自噬在白念珠菌耐药中的作用。方法实时荧光定量PCR(quantitative real-time PCR,RT-qPCR)比较白念珠菌耐药株与标准株自噬相关基因表达,ALP活性检测比较两组自噬水平,透射电镜观察耐药株与标准株的自噬现象。结果RT-qPCR显示白念珠菌耐药株与标准株自噬相关基因表达存在明显差异;ALP活性检测表明耐药株自噬活性较标准株高;透射电镜观察到耐药株中颗粒状的自噬小体。结论白念珠菌浮游状态耐药株与标准株自噬存在差异,提示耐药性的产生可能存在自噬的调节。

Objective To compare the differences of autophagy between drug-resistant strains and standard strains of planktonic Candida albicans,and to explore the role of autophagy in drug resistance in Candida albicans.Methods Quantitative real-time PCR(RTqPCR)was used to detect the expression of autophagy-related genes between the drug-resistant strains and the standard strains.Alkaline phosphatase(ALP)activity detection was performed to evaluate the level of autophagy between the two strains.The autophagosomes of the strains was observed by transmission electron microscopy(TEM).Results RT-qPCR results showed that the expression of autophagy-related genes was significantly different between the drug-resistant strains and the standard strains.ALP activity showed that compared with standard strains,the autophagy activity of the drug-resistant strains increased significantly.Autophagosomes were observed in drug-resistant strains under the transmission electron microscopy.Conclusion The autophagy of the drug-resistant strains of Candida albicans is different from that of the standard strains and the emergence of drug resistance in Candida albicans may be regulated by autophagy.