通胆汤对原发性胆汁性胆管炎模型小鼠Mac-2BP表达的影响

Effect of Tongdan Decoction on Mac-2BP Expression in Primary Niliary cholangitis Model Mice112

【目的】探讨通胆汤对原发性胆汁性胆管炎(PBC)模型小鼠的干预作用及机制。【方法】将Mac-2结合蛋白(Mac-2BP)基因敲除小鼠(作为A组)与野生型C57BL/6小鼠(作为B组),采用2-辛炔酸结合牛血清白蛋白(2OA-BSA)腹腔注射构建PBC模型。8周后评价造模是否成功。将造模成功的A组小鼠再随机分为A1、A2组,B组随机分为B1、B2组。A1、B1组给予磷酸盐缓冲液(PBS)灌胃为对照;A2、B2组给予通胆汤灌胃。连续治疗12周后,观察小鼠肝组织病理变化、肝功能指标及肝组织Mac-2BP的蛋白和mRNA表达水平。【结果】应用2OA-BSA构建PBC小鼠模型8周,可得到在病理及生化学上与人类PBC相类似的改变。经通胆汤干预后,B2组生化学指标较A2组改善得更为明显;B2组肝组织Mac-2BP蛋白和mRNA表达量较B1组明显降低(P<0.01)。【结论】通胆汤可改善PBC模型小鼠的胆汁淤积情况,与其下调Mac-2BP的蛋白和mRNA表达相关,但其具体机制有待进一步研究。

Objective To explore the effect of Tongdan Decoction on Mac-2 binding protein(Mac-2 BP)expression in primary biliary cholangitis(PBC)model mice. Methods Mac-2 BP knockout C57 BL/6 mice(as group A)and wild-type C57 BL/6 mice(as group B)were induced into PBC model by intraperitoneal injection of 2-octynoic acid coupled to BSA(2 OA-BSA). After 8 weeks,it was evaluated whether the modeling was successful or not. And then,the successful modeled PBC mice were randomly divided into group A and group B. Group A was randomly divided into group A1 and group A2,and group B was randomly divided into group B1 and group B2. Groups A1 and B1 were given intragastric administration of phosphate buffer solution(PBS),and groups A2 and B2 were given intragastric administration of Tongdan Decoction. After 12 weeks of treatment,the pathological features of liver tissue,liver function index and Mac-2 BP protein and m RNA expression level in liver tissue were observed.Results Using 2 OA-BSA to establish a PBC mouse model for 8 weeks,the changes obtained in pathology and biochemistry were similar to human PBC. After Tongdan Decoction treatment,the improvement in biochemical parameters of group B2 was more obvious than that of group A2;the protein and mRNA expression content of Mac-2 BP in group B2 was significantly lower than that of group B1(P<0.01). Conclusion Tongdan Decoction has effects on improving cholestasis in PBC model mice,which is related to its down-regulation of Mac-2 BP protein and mRNA expression,but its specific mechanisms needs further study.