聚维酮碘对角膜上皮细胞氧化损伤的影响

Oxidative damage effect of povidone-iodine on corneal epithelial cells

目的:观察聚维酮碘对角膜上皮细胞氧化损伤的影响。方法:观察不同浓度聚维酮碘对角膜上皮细胞氧化损伤的影响,取体外培养的生长良好的角膜上皮细胞按随机数字法分为:对照组、低浓度组、中浓度组和高浓度组。观察不同消毒时间聚维酮碘对角膜上皮细胞氧化损伤的影响,取体外培养的生长良好的角膜上皮细胞按随机数字法分为:对照组、短时间组、中等时间组和长时间组。采用ELISA检测各组丙二醛(MDA)、超氧化物歧化酶(SOD)含量,采用倒置显微镜、CCK-8法检测各组细胞活性,采用流式细胞仪检测各组细胞的凋亡率。结果:聚维酮碘浓度越高,角膜上皮细胞的MDA含量越高,SOD含量越低,细胞活性越低,凋亡率越高。聚维酮碘对角膜上皮细胞的氧化损伤呈浓度依赖性,组间有差异(均P<0.01)。聚维酮碘消毒时间越长,角膜上皮细胞的MDA含量越高,SOD含量越低,细胞活性越低,凋亡率越高。聚维酮碘对角膜上皮细胞的氧化损伤呈时间依赖性,组间有差异(均P<0.01)。结论:聚维酮碘对角膜上皮细胞有氧化损伤作用,损伤呈浓度和时间依赖性。

AIM:To investigate the oxidative damage effect of povidone iodine on corneal epithelial cells.METHODS:To study the oxidative damage effect of different concentrations of povidone iodine,the cultured epithelial cells were randomly divided into control group,low concentration group,medium concentration group and high concentration group.To study the oxidative damage effect of disinfection time of povidone iodine,the cultured corneal epithelial cells were randomly divided into control group,short time group,medium time group and long time group.Malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by ELISA,cell viability was detected by CCK-8 method and inverted microscope,and apoptotic rate was detected by flow cytometry.RESULTS:The higher concentration of povidone iodine was associated with the higher MDA content,the lower SOD content,the lower cell activities and the higher apoptotic rate of the corneal epithelial cells,which was in a dose-independent manner.The differences among four groups were statistically significant(all P<0.01).The longer disinfection time of povidone iodine was related with the higher MDA content,the lower SOD content,the lower cell activities and the higher apoptotic rate of corneal epithelial cells,which was in a time-independent manner.The differences among four groups were statistically significant(all P<0.01).CONCLUSION:The oxidative damage of povidone iodine on corneal epithelial cells were in a dose independent and time dependent manner.