miR-429和Bmi-1mRNA在肺癌组织中的表达及其临床意义

Clinical significance of miR-429 and Bmi-1 mRNA expressions in lung carcinoma tissues

目的:研究miR-429和Bmi-1 mRNA在肺癌(LC)组织中的表达及其与患者临床特征、预后之间的关系,并探讨其表达水平对LC患者预后的预测意义。方法:选取2013年1─12月在河北医科大学第四医院就诊的60例LC患者癌组织及癌旁组织,实时荧光定量PCR(qPCR)技术检测组织中miR-429及Bmi-1 mRNA的表达水平,并分析二者之间表达水平的关系及其与患者临床病理指标及预后之间的关系。结果:与癌旁组织比较,LC组织中miR-429表达水平明显降低,而Bmi-1 mRNA的表达水平明显升高(P<0.01)。LC组织中miR-429低表达而Bmi-1 mRNA高表达的患者往往瘤组织较大、临床分期较晚、更易发生淋巴结转移,同时患者生存期较短。结论:LC组织中低表达miR-429及高表达Bmi-1 mRNA可能是患者临床进展及预后较差的预测指标。

OBJECTIVE:To study the clinical significance of the expression of miR-429 and Bmi-1 in lung carcinoma(LC)tissues.METHODS:Samples of tumor tissues and paraplastic tissues were collected from 60 LC patients,January to December,2013.Expression of miR-429 and Bmi-1 mRNA in both tissues were detected by the quantitative real-time PCR(qPCR)assay.In addition,their relationships with clinical features and prognosis of the patients were analysed.RESULTS:The tumor tissues showed significantly decreased expression of miR-429 and increased expression of Bmi-1,compared with the paraplastic tissues(P<0.01).Among the patients,those with lower expression of miR-429 and higher expression of Bmi-1 in their tumor tissues tended to have larger tumors,later clinical stages,more prone to lymph node metastasis and shorter survival time.CONCLUSION:The results show that the LC patients with lower miR-429 expression and/or higher Bmi-1 expression in tumour tissues frequently had worse clinical outcomes and poorer prognoses.