白细胞介素18单核苷酸多态性与73例喉鳞状细胞癌易感性的关联性

Correlation between interleukin-18 single nucleotide polymorphism and susceptibility of laryngeal squamous cell carcinoma: a report of 73 cases

目的探讨白细胞介素18(IL-18)启动子位点-137G/C和-607C/A基因多态性与山东地区汉族人群喉鳞状细胞癌(LSCC)易感性的关系。方法随机选取2017年5月至2019年10月就诊于临沂市人民医院的喉鳞状细胞癌患者73例为病例组,选取同期查体的健康志愿者73人为健康对照组。收集研究对象一般临床资料及外周静脉血2 mL,提取全血DNA,采用sanger测序法进行基因测序及SNP分型。所有定量资料采用Student?s-t检验分析,定性资料采用Pearson χ~2检验或Fisher精确检验,应用卡方拟合优度检验分析健康对照组是否符合Hardy-Weinberg平衡定律,采用二元Logistic回归分析计算两种单核苷酸多态性(SNP)位点以及各风险因素(年龄、吸烟、饮酒)与LSCC发病风险的关联性。结果与健康对照组相比,病例组中-137G/C位点变异基因型GC的基因型频率升高(34.2%vs 19.2%,P=0.040),而-607C/A位点各基因型频率的分布在病例组和健康对照组之间差异无统计学意义(P>0.05)。吸烟及饮酒均会升高LSCC的发病风险(OR分别为5.710和3.116)。对基因型、年龄组、吸烟史及饮酒史4种风险因素进行调整后,吸烟者与-137G/C基因型携带者LSCC发病风险的OR值分别为4.967(95%CI=2.185~11.289,P<0.001)和2.971(95%CI=1.243~7.101,P=0.014),差异有统计学意义。结论 IL-18启动子位点-137G/C而非-607C/A的多态性与LSCC的患病风险升高有关联性。

Objective To investigate the correlation between interleukin-18(IL-18) and promotors-137 G/C and-607 C/A gene polymorphisms and the susceptibility of laryngeal squamous cell carcinoma(LSCC) in a Chinese Han population in Shandong Province. Methods A total of 73 LSCC patients(case group) treated at Linyi People?s Hospital during May 2017 and Oct. 2019 and 73 healthy controls were enrolled. The clinical information and peripheral venous blood samples(2 mL) were collected. The whole blood DNA was extracted, and gene sequencing and SNP genotypingwere conducted. The quantitative data were analyzed with Student?s-t test, and the qualitative data were analyzed with Pearson χ~2 test or Fisher exact test. The control group was analyzed with chi-square goodness of fit test to verify whether it was in accordance with the Hardy-Weinberg equilibrium law. The correlation between two SNP loci and risk factors(age, smoking, drinking) with LSCC risk was determined with binary Logistic regression analysis. Results The case group had higher genotype frequency of GC at-137 G/C(34.2% vs 19.2%, P=0.040), but there was no significant difference in the genotype frequency at-607 C/A between the two groups(P>0.05). Smoking and drinking increased the risk of LSCC(OR=5.710;3.116). After the risk factors of genotype, age, smoking and drinking history were adjusted, the OR of LSCC risk in smokers and-137 G/C carriers were 4.967(95%CI=2.185-11.289, P<0.001) and 2.971(95%CI=1.243-7.101, P=0.014), respectively. Conclusion Our findings suggest that IL-18 promoter-137 G/C polymorphism is correlated with an increased risk of LSCC.