Development of a PCR-based method to monitor arthropod dispersal in agroecosystems:Macrolophus pygmaeus(Hemiptera:Miridae)from banker plants to tomato crops

Development of conservation biological control programs requires the identifi-cation of sources that contribute to predator colonization of crops.Macrolophus pygmaeus(Rambur)(Hemiptera:Miridae)is an efficient polyphagous predator used in biological con-trol programs in vegetable crops in Europe.We have developed a marking method based on spraying with a solution of the brine shrimp Artemia spp.(Anostraca:Artemidac)cysts,followed by a PCR detection of Artemia DNA to monitor M.pygmaeus dispersal from banker plants to tomato crops.Experiments conducted in climatic chambers show that the topical application of this marking solution on M.pygmaeus does not significantly reduce adult longevity and that it is detected up to 6 d after the application.When this Artemia solution was applied on Calendula officinalis L.banker plants harboring M.pygmaeus and maintained outdoors,Artemia DNA was still detected on 62%of the insects after6 d.The conducted field applications in commercial greenhouses have confirmed the usefulness of this method to monitor M.pygmaeus dispersal from banker plants to a newly planted tomato crop.This method can be used to assess arthropod movement,being an interesting molecular approach for further improving future pest management strategies.