细菌瓶法用于产甲烷菌菌量的测定

Determination of Methane-producing Bacteria Using Bacterial Bottle Method

在微生物采油(MEOR)研究、废水厌氧生物处理及沼气发酵等领域,产甲烷菌(MPB)菌量的测定目前主要采用的是繁琐的Hungate滚管培养法。为了更准确、简便测定MPB的菌量。推荐使用含有碳源、氮源、磷源及化学脱氧剂等的培养液MPB专用测试瓶。将待测水样注入测试瓶逐级稀释,在30℃或现场温度±5℃培养14 d后,培养液变浑浊并出现气泡表示有MPB生长。MPB的计数按常规细菌瓶法进行。用所推荐的细菌瓶法测定了油田采出液、微生物采油菌剂、污水处理厂厌氧消化池中MPB的菌量,所得结果与Hungate滚管培养法结果相同。与Hungate滚管培养法相比,细菌瓶法生长指示明显、操作简单,效率高。

In the field of microbial enhanced oil recovery(MEOR)research,anaerobic biological wastewater treatment and biogas fermentation,the amount of methane-producing bacteria(MPB)was determined by the complicated Hungate roller culture method.In order to measure the amount of MPB more easily and accurately,it was recommended to use a MPB-specific test bottle containing carbon source,nitrogen source,phosphorus source and chemical deoxidizer.The results showed that the water sample was infused into the test bottle and was diluted step by step,the culture solution became cloudy and bubbles appeared after culturing for 14 days at 30℃or on-site temperature±5℃,indicating the growth of MPB.The MPB count was performed according to the conventional bacterial bottle method.The amounts of MPB in oilfield production fluid,microbial enhanced oil recovery agents and sewage treatment plant anaerobic digestion tank were determined by the bacterial bottle method,and the results were basically the same as those by the Hungate roller culture method.Compared with the Hungate roller culture method,the bacterial bottle method had the advantages of obvious growth indication,simple operation and high work efficiency.