响应面法优化侧柏叶总黄酮提取工艺

Optimization of Extraction Process of Total Flavonoids from Platycladus Orientalis Leaves by Response Surface Methodology

利用超声波辅助提取侧柏叶总黄酮,用分光光度法分析其含量。研究了乙醇浓度、超声提取时间、液料比对侧柏叶总黄酮提取的影响。在单因素实验的基础上,以侧柏叶总黄酮提取率为响应值,以乙醇浓度、超声提取时间、液料比为自变量,通过三因素三水平Box-Bohnken响应面分析法对筛选出的提取工艺进行优化。结果表明,响应面法优化得到的最佳提取工艺为:在乙醇浓度73%、提取时间42 min、液料比为80∶1 mL/g,此实验条件下重复3次验证实验得到的侧柏叶总黄酮平均提取率为2.45%,与Box-Bohnken响应面分析法模拟预测值2.47%相差较小,所建模型与采用的优化方法可靠。

The total flavonoids of Platycladus orientalis leaves were extracted with the aid of ultrasound,and their contents were analyzed by spectrophotometry.The effects of ethanol concentration,ultrasonic extraction time and liquid-to-material ratio on the extraction of total flavonoids in Platycladus orientalis leaves were investigated.Based on the single factor experiment,the total flavonoids extraction rate of Platycladus orientalis leaves was taken as the response value,and the ethanol concentration,ultrasonic extraction time,and liquid-to-material ratio were used as independent variables.The optimized extraction process was investigated.The optimal extraction process obtained by response surface method optimization was ethanol concentration of 73%,extraction time of 42 min,and a liquid-to-material ratio of 80∶1 mL/g.On this condition repeating the verification experiment three times under obtain the total flavonoids extraction rate of Platycladus orientalis leaves is 2.45%,which is near value of 2.47%from the Box-Bohnken response surface analysis simulation prediction.The model established is reliable with the optimization method used.