摘要
目的分析COVID-19确诊病例住院期间和出院后不同类型标本中病毒核酸的检出情况。方法调查定点医院收治的COVID-19确诊病例,住院期间采集其鼻咽拭子、粪便、尿液和血液标本,出院后进行随访,采集其鼻咽拭子和粪便标本,采用实时荧光RT-PCR方法进行核酸检测。结果共调查25例COVID-19确诊病例,在住院期间采集其鼻咽拭子、粪便、尿液和血液标本各25份,共100份标本,经检测鼻咽拭子均为病毒核酸阳性,住院期间9例患者粪便中检出核酸阳性,阳性率为36.00%(9/25),尿液和血液未检出病毒核酸。25例患者出院后进行随访,在第7天和第14天采集其鼻咽拭子和粪便标本各25份,第7天2例患者检出鼻咽拭子核酸阳性,复阳率为8.00%(2/25),粪便中未检出病毒核酸,出院后第14天鼻咽拭子和粪便中均未检出病毒核酸。结论COVID-19确诊病例住院治疗期间粪便中可检出病毒核酸,出院后部分患者鼻咽拭子病毒核酸复阳。
Objective To investigate the positive rates of 2019-nCoV nucleic acid in different specimens from confirmed COVID-19 cases during hospitalization and after discharge.Methods Patients with confirmed COVID-19 were enrolled from designated hospitals.Nasal swabs,throat swabs,and specimens of stool,urine and blood were collected during hospitalization.After the patients were discharged,nasal swabs,throat swabs and stool specimens were collected during follow-up.Real-time RT-PCR was used to detect 2019-nCoV nucleic acid.Results This study involved 25 confirmed COVID-19 cases.During hospitalization,all patients tested positive in both nasal and throat swab 2019-nCoV nucleic acid tests,and nine of them(36.00%)were positive in stool specimen test.Urine and blood specimen test results were all negative.Nasal swabs,throat swabs and stool specimens were collected from each patient 7 d and 14 d after discharge.Two patients(8.00%)tested positive for 2019-nCoV nucleic acid again in nasal and throat swab tests on 7 d,while all stool specimen tests were negative.No 2019-nCoV nucleic acid was detected in nasal swabs,throat swabs or stool samples on 14 d.Conclusions 2019-nCoV nucleic acid was detected in stool samples of confirmed COVID-19 cases during hospitalization.Nasal and throat swab nucleic acid tests turned positive again in some patients after discharge.
作者
薛泽润
尤海菲
栾阳
陈海龙
杜全丽
韩月雯
樊宁秀
杨玉捷
邓杨妮
李凯欣
党丽云
马超锋
吴瑞
Xue Zerun;You Haifei;Luan Yang;Chen Hailong;Du Quanli;Han Yuewen;Fan Ningxiu;Yang Yujie;Deng Yangni;Li Kaixin;Dang Liyun;Ma Chaofeng;Wu Rui Viral(Diseases Laboratory,Xi′an Municipal Center for Disease Control and Prevention,Xi′an 710054,China;Institute of AIDS/STD Control and Prevention,Shaanxi Provincial Center for Disease Control and Prevention,Xi′an 710054,China;Department of Immunization Program,Shiquan County Center for Disease Control and Prevention,Ankang 725200,China;Tuberculosis Internal Medicine Department,Xi′an Chest Hospital,Xi′an 710100,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2020年第8期569-573,共5页
Chinese Journal of Microbiology and Immunology
基金
西安市新型冠状病毒肺炎疫情应急防治科技专项(20200003YX003-1,20200001YX001-2)。
