二硫化钼纳米材料的制备及其对心肌细胞的保护作用

Preparation of Molybdenum Disulfide Nanoparticles and the Cytoprotection on Cardiac Myocytes

目前,心血管疾病是世界上导致死亡的主要病因,而氧化应激损伤是心血管疾病的重要发病机制之一。本研究旨在探讨二硫化钼(MoS 2)纳米材料在心肌细胞受到氧化应激损伤时的抗氧化保护作用。我们通过一步水热法合成了具备类抗氧化酶活性的MoS 2纳米材料。通过MTT比色法、细胞内凋亡、细胞内活性氧(ROS)检测及Western Blot等实验证实MoS 2纳米材料(100μg/mL)可以很好地保护H9c2心肌细胞免受双氧水(H2O2)诱导的氧化应激损伤。同时,还促进了H9c2心肌细胞的增殖。本研究显示了MoS2纳米材料可用于构建针对氧化应激损伤引起的心血管疾病的抗氧化剂防御,为纳米药物在抗心肌氧化损伤研发提供实验数据及理论依据。

Cardiovascular diseases currently dominate the cause of death worldwide.Oxidative stress injury is one of the important pathogenesis of cardiovascular diseases.The purpose of this study is to investigate the antioxidative protection effect of molybdenum disulfide(MoS2)nanoparticles on cardiomyocytes under oxidative stress injury.We synthesized MoS 2 nanoparticles with antioxidant-like enzyme activity by a one-step hydrothermal method.MTT,intracellular apoptosis,intracellular reactive oxygen species(ROS)detection,and Western Blot experiments confirm that MoS 2 nanoparticles(100μg/mL)can protect H9c2 cardiomyocytes from oxidative stress injury induced by hydrogen peroxide(H2O2).At the same time,it also promotes the proliferation of H9c2 cardiomyocytes.This study shows that MoS 2 nanoparticles can be used to construct antioxidant defense against cardiovascular diseases caused by oxidative stress injury,and provide experimental data and theoretical basis for the development of nanomedicine in the resistance to myocardial oxidative damage.