乳腺浸润癌中HER-2基因扩增与蛋白表达相关性分析

Correlation analysis of HER-2 gene amplification and protein expression in invasive breast cancer

目的比较分析乳腺浸润癌患者中HER-2蛋白表达与基因扩增的差异性和相关性。方法利用免疫组织化学(IHC)与荧光原位杂交(FISH)技术分别检测乳腺浸润癌患者HER-2蛋白表达和基因扩增情况,并对结果进行相关性分析。结果乳腺浸润癌中HER-2蛋白表达及基因扩增结果具有较好的一致性,总体一致率为91%,IHC结果(+++)中二者结果一致率最高,结果(++)中二者结果一致率最低。结论FISH与IHC两项技术在检测HER-2基因扩增及蛋白表达方面具有较好的一致性。乳腺癌患者应常规进行HER-2的IHC检查,当IHC阳性结果不明确时需进一步行FISH检测HER-2基因的扩增情况,从而为临床综合治疗方案提供更可靠的依据。

Objective Comparative analysis of the differences and correlations between HER-2 protein expression and gene amplification in patients with invasive breast cancer.Methods Immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)techniques were used to detect the expression of HER-2 protein and gene amplification in patients with invasive breast cancer,and the results were analyzed for correlation.Results HER-2 protein expression was consistent with gene amplification in invasive breast cancer,with 91%overall agreement.The IHC result(+++)showed the highest agreement rate,and the(++)result showed the lowest agreement rate.Conclusion The two technologies,FISH and IHC,have good consistency in detecting the gene amplification and protein expression of the HER-2 gene.HER-2 protein level should be detected by IHC for breast cancer patients.When the results of IHC are not clear,further FISH should be used to detect the amplification of HER-2 gene,so as to provide a more reliable basis for clinical comprehensive treatment programs.