影响意大利水牛精液品质的相关因素分析

Analysis of Related Factors in Semen Quality of Italian Buffaloes

本研究旨在通过对意大利水牛精液品质分析、睾丸周径测量、精浆中的氧化应激水平检测和精子活力相关基因表达情况来探究影响精液质量的相关因素。试验检测了6头意大利水牛精液的活力、畸形率、采精量,并在测量其阴囊周径后进行相关性分析;检测了意大利水牛精浆中氧化应激水平指标(丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px))并进行了相关性分析;分析了意大利水牛精子β-微管蛋白-2c(TUBB2C)、外周致密纤维2(ODF2)、筑丝蛋白2(TEKT2)、筑丝蛋白4(TEKT4)基因的表达定量及相关性。结果显示,意大利水牛阴囊周径与精液产量、活力、畸形率之间相关系数分别为0.423(P>0.05)、0.750(P<0.01)、-0.827(P<0.01),即阴囊周径与精子活力呈显著正相关关系、与畸形率呈显著负相关关系;意大利水牛精子活力与MDA、T-SOD、GSH-Px指标之间相关系数分别为-0.522(P<0.05)、0.333(P>0.05)、0.474(P<0.05),即精子活力与MDA含量之间存在显著负相关关系,与GSH-Px活性之间存在显著正相关关系,与T-SOD活性相关性不显著;意大利水牛精子活力与TUBB2C、ODF2、TEKT2、TEKT4基因指标之间相关系数分别为0.930(P<0.01)、0.726(P<0.01)、0.924(P<0.01)、0.839(P<0.01),即精子活力与以上基因表达量存在显著正相关关系。本研究结果为了解影响意大利水牛精液质量的因素提供参考,也为意大利种公水牛的筛选提供理论依据。

This study was aimed to investigate the factors affecting semen quality by analyzing the semen quality of Italian buffaloes,measuring the circumference of the testis,the level of oxidative stress in seminal plasma and the expression of genes related to sperm motility.In this study,the sperm vitality,deformity rate and semen collection of 6 Italian buffaloes were tested,and the correlation analysis was carried out after measuring scrotal circumference.The oxidative stress level of seminal plasma in Italian buffaloes was tested,including malondialdehyde(MDA),total superoxide dismutase(T-SOD)and glutathione peroxidation(GSH-Px).The expression ofβ-tublin-2c(TUBB2C),outer dense fibres 2(ODF2),tektin-2(TEKT2),tektin-4(TEKT4)genes and their correlation with sperm in Italian buffaloes were analyzed.The results showed that the correlation between scrotal circumference and semen yield,vigor and deformity rate was found,the coefficients were 0.423(P>0.05),0.750(P<0.01)and-0.827(P<0.01),which showed a significant positive correlation between scrotal circumference and sperm motility,and a significant negative correlation with the deformity rate.The oxidative stress level in seminal plasma of Italian buffaloes was tested,the correlation coefficients of MDA,T-SOD and GSH-Px with sperm motility were-0.522(P<0.05),0.333(P>0.05)and 0.474(P<0.05),respectively.There was a significant negative correlation between sperm motility and MDA content,a significant positive correlation between GSH-Px activity and sperm motility,no significant correlation with T-SOD activity.The correlation coefficients between sperm motility and TUBB2C,ODF2,TEKT2 and TEKT4 genes were 0.930(P<0.01),0.726(P<0.01),0.924(P<0.01)and 0.839(P<0.01),respectively.There was a significant positive correlation between sperm motility and the expression of four genes.The results provided a reference for understanding the factors affecting the semen quality of Italian buffaloes,and also provided a theoretical basis for the selection of Italian bull.