AA9家族裂解多糖单加氧酶研究进展

Progress of AA9 Family Lytic Polysaccharide Monooxygenase

AA9家族裂解多糖单加氧酶(LPMO)通过氧化裂解破坏纤维素的结晶结构,提高其酶水解效率,在生物炼制行业应用前景广阔。综述了AA9家族LPMO对纤维素酶水解木质纤维素的活性促进作用;归纳了AA9家族LPMO在外源电子供体及氧气的参与下对糖苷键进行氧化裂解的反应机制,并对其外源电子供体系统进行了合理的分类;对AA9家族LPMO的活性测定方法及重组表达技术进行了探讨;最后对AA9家族LPMO有待深入开展的研究内容进行了展望,以期为AA9家族LPMO的研究和应用提供参考。

AA9 family lytic polysaccharide monooxygenase(LPMO)can destroy the crystalline structure of cellulose through oxidative cleavage of the glycosidic bonds in the glucan chain,improving the hydrolysis efficiency of cellulase toward crystalline cellulose,and thus having a broad application prospect in secondary biorefinery industry.In this paper,the boosting effect of AA9 family LPMO on cellulase hydrolysis of lignocellulose was reviewed.The reaction mechanism of oxidative cleavage of glycosidic bond by AA9 family LPMO with the participation of exogenous electron donor and oxygen was summarized,and the exogenous electron donor system thereof was reasonably classified.The activity determination method and recombinant expression technology of AA9 family LPMO were discussed.Finally,the further important research contents of AA9 family LPMO are prospected,in hope of providing reference for the research and application of AA9 family LPMO.