ANRIL对胃癌细胞增殖的影响

Effect of ANRIL on proliferation of gastric cancer cells

目的:研究在胃癌细胞系SGC-7901中,ANRIL对其增殖的影响,并探讨该作用是否与TGF-β/Smad通路相关。方法:采用小干扰RNA(small interfering RNA,siRNA)技术对SGC-7901细胞中的ANRIL基因、TGF-β1基因分别进行干预,并对ANRIL基因及TGF-β1基因进行联合干预;应用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法检测各实验组中细胞增殖能力的变化;采用Western blot法检测各实验组中TGF-β1蛋白和p-Smad蛋白的表达情况。结果:胃癌细胞系SGC-7901经转染24 h、48 h后,siANRIL组OD值明显低于NC组、MOCK组及BLANK组(P<0.05)。siANRIL+siTGF-β1组、siTGF-β1组OD值高于NC组、MOCK组及BLANK组(P<0.05)。siANRIL+siTGF-β1组OD值介于siANRIL组与siTGF-β1组之间(P<0.05)。siANRIL组TGF-β1蛋白和p-Smad蛋白的表达高于NC组、MOCK组及BLANK组(P<0.05)。在siTGF-β1组及siANRIL+siTGF-β1组中TGF-β1蛋白和p-Smad蛋白的表达低于NC组、MOCK组及BLANK组(P<0.05)。siANRIL+siTGF-β1组TGF-β1蛋白和p-Smad蛋白的表达介于siANRIL组与siTGF-β1组之间(P<0.05)。结论:在胃癌细胞系SGC-7901中,ANRIL对其细胞增殖发挥促进作用,且ANRIL可能是通过TGF-β/Smad信号传导通路发挥促进作用的。

Objective:To study the effect of ANRIL on the proliferation of gastric cancer cell line SGC-7901,and to explore whether this effect is related to the TGF-β/Smad pathway.Methods:Small interfering RNA(siRNA)technology was used to interfere with ANRIL gene and TGF-β1 gene in SGC-7901 cells,and combined with ANRIL gene and TGF-β1 gene.Methyl thiazolyl tetrazole(MTT)assay was used to detect the changes of cell proliferation ability in each experimental group,and Western blot was used to detect the expression of TGF-β1 and p-Smad proteins in each experimental group.Results:After 24 and 48 hours of transfection,MTT results showed that the OD value of siANRIL group was significantly lower than that of NC group,MOCK group and BLANK group in SGC-7901 cell line(P<0.05).Compared with NC group,MOCK group and BLANK group,the OD value of siANRIL+siTGF-β1 group and siTGF-β1 group augmented(P<0.05).The OD value of siANRIL+siTGF-β1 group was between that siANRIL group and siTGF-β1 group(P<0.05).The expressions of TGF-β1 and p-Smad protein in siANRIL group were significantly higher than those in NC group,MOCK group and BLANK group(P<0.05).The expression of TGF-β1 and p-Smad protein in siTGF-β1 group and siANRIL+siTGF-β1 group were significantly lower than those in NC group,MOCK group and BLANK group(P<0.05).Contrasted with NC group and BLANK group,the expressions of TGF-β1 and p-Smad protein in siANRIL+siTGF-β1 group was between that in siANRIL group and that in siTGF-β1 group(P<0.05).Conclusion:In gastric cancer cell line SGC-7901,ANRIL promotes cell proliferation,and ANRIL may play a role through the TGF-β/Smad signal transduction pathway.