体外模拟动态力学刺激对SD幼鼠前脂肪细胞增殖及凋亡的影响研究

Effects study of simulated dynamic mechanical stimulation in vitro on proliferation and apoptosis of preadipocytes in SD rats

目的探讨体外模拟动态力学刺激对SD幼鼠前脂肪细胞增殖及凋亡的影响,为推拿按摩治疗青少年单纯性肥胖的现代医学细胞生物学机制提供理论与实验依据。方法体外培养SD幼鼠前脂肪细胞,从细胞生物力学角度模拟推拿治疗的按摩作用方式,对前脂肪细胞实施不同程度动态力学刺激(0、1.5、3.0 Hz),观察动态力学刺激对前脂肪细胞增殖及凋亡的影响。结果1.5 Hz组及3.0 Hz组前脂肪细胞的增殖活性较0 Hz组降低(1.5 Hz组P=0.019;3.0 Hz组P=0.006),而1.5 Hz组及3.0 Hz组细胞的凋亡与0 Hz组比较差异无统计学意义(1.5 Hz组P=0.111;3.0 Hz组P=0.091)。结论推拿按摩可能通过抑制前脂肪细胞的增殖而达到减肥的效果。

Objective To investigate the effect of simulated dynamic mechanical stimulation in vitro on the proliferation and apoptosis of preadipocytes in SD young rats,and to provide theoretical and experimental basis for the treatment of simple obesity in adolescents with massage.Methods The preadipocytes of SD young rats were cultured in vitro,and the action mode of massage therapy was simulated from the perspective of cell biomechanics.Different degrees of dynamic mechanical stimulation(0,1.5,3.0 Hz)were applied to the preadipocytes.The effects of dynamic mechanical stimulation on the proliferation and apoptosis of preadipocytes were observed.Results The proliferative activities of preadipocytes in the 1.5 Hz group and the 3.0 Hz group were higher than that in the 0 Hz group(P=0.019 in the 1.5 Hz group;P=0.006 in the 3.0 Hz group).There were no statistically significant differences in apoptosis rate of the 1.5 Hz group and the 3.0 Hz group when compared with the 0 Hz group(P=0.111 in the 1.5 Hz group;P=0.091 in the 3.0 Hz group).Conclusion Massage therapy may achieve weight loss by inhibiting the proliferation of pre adipocytes.