高效液相色谱法检测猪肉、羊肉中阿托品残留量的研究

Study on the Determination of Atropine Residues in Pork and Lamb by HPLC

为了检测阿托品在猪肉、羊肉中残留量,本研究通过实验条件的建立与优化,建立了其高效液相色谱分析方法。猪肉、羊肉中的阿托品经乙腈提取两次,用正己烷除脂两次,再经HLB柱净化后,用高效液相色谱仪分离,紫外检测器检测,外标法定量。结果表明:阿托品在0.5~20.0μg/mL的系列浓度范围内均呈良好线性关系,相关系数r均大于0.999。样品中阿托品的检测限为0.25 mg/kg,定量限为0.5 mg/kg。在0.5~5 mg/kg添加浓度范围内,阿托品的回收率均为80~110%,相对标准偏差(RSD)均小于10%。该方法具有简便快捷、灵敏度高、定性准确等特点。

High performance liquid chromatography(HPLC)method by the established and optimized conditions for the determination of atropine residues in pork and lamb.Pork and lamb samples were extracted twice with acetonitrile,degreased twice with n-hexane,and were cleared up by HLB column.Quantification of the analyte was achieved by HPLC using external standard.The results indicated that the correlations were greater than 0.999 at the range of 0.5 to 20.0μg/mL.The limit of detection of atropine was 0.25 mg/kg,and the limit of quantification was 0.5 mg/kg.The average recoveries of atropine ranged from 80%to 110%at spiled levels of 0.5 to 5 mg/kg with RSD less then 10%.The experiment show that the method is simple,rapid,high sensitivity,good reproducibility and suitable for detection of atropine residues in pork and lamb.