血管生成素-2上调增强血管瘤干细胞血管形成能力的研究

Increased Expression of Angiopoietin-2 Enhanced Angiogenesis of Hemangioma Stem Cells

目的:探究Ang2上调后对血管瘤干细胞(HemSCs)血管形成能力的影响,揭示Ang-Tie2体系在血管瘤血管发生发展过程中的作用。方法:通过免疫磁珠阳性分选技术分离纯化HemSCs,经体外培养扩增,通过慢病毒转染的方法获得Ang2上调稳定表达的HemSCs。通过血管形成实验检测Ang2上调的HemSCs血管形成能力的变化,通过WesternBlot检测相关细胞信号通路关键靶蛋白表达变化。结果:Ang2上调的HemSCs血管形成能力大于对照组;血管生成信号通路关键靶蛋白在HemSCs Ang2上调组中表达增强。结论:Ang2上调可以增强HemSCs的血管形成能力。

Objective To investigate the effect of Ang2 up-regulation on the Angiogenic capacity in hemangioma stem cells(HemSCs)and to reveal the role of Ang-Tie2 system in the vasculogenesis of hemangiomas.Methods Separation and purification of HemSCs by positive selected immunomagnetic beads sorting.After being cultured and amplified in vitro,upregulation of Ang2 by stable lentiviral transfection HemSCs.The vasculogenesis ability of HemSCs cells up-regulated by Ang2 was observed by Angiogenesis Experiment.Detect the expression changes of key protein target in cell signaling pathway by Western Blot Protein.Results Ang2 up-regulated HemSCs angiogenesis ability was greater than the control group.Key target proteins of angiogenesis signaling pathway was enhanced in HemSCs Ang2 up-regulated.Conclusion Ang2 up-regulation can enhance HemSCs'angiogenesis ability.