羊肚菌菌丝体对土壤酶活性的影响

Effect of Morchella Mycelium on Soil Enzymatic Activity

探究羊肚菌菌丝体对土壤酶活性的影响。通过单因素和正交试验,采用3,5-二硝基水杨酸比色法测定羊肚菌菌丝体对土样中蔗糖酶和淀粉酶活性的影响。结果显示,菌土影响土壤蔗糖酶活性最佳条件为温度28℃,时间3 d,菌丝体添加量为1∶5,土壤蔗糖酶活性增加为对照组的12倍;土壤淀粉酶活性的最佳条件为时间7 d,菌丝体添加量1∶5,温度28℃,土壤淀粉酶活性增加为对照组的18倍。在短期内羊肚菌菌丝体能提高土壤中蔗糖酶和淀粉酶活性,对改良土壤具有重要意义。

This work aims to study on the effect of Morchella mycelium on soil enzymatic activity.The sucrase and amylase activities of soil samples were determined by 3,5-dinitrosalicylic acid colorimetric method with single factor and orthogonal experimental design.The optimal condition of the soil sucrase activity was that the temperature was 28℃,the time was 3 d,the amount of added mycelium was 1∶5,and the soil sucrase activity increased 12 times compared with the control group.The optimal condition of soil amylase activity was as such:the amount of added mycelium was 1∶5,the temperature was 28℃,the time was 7 d,and the soil amylase activity increased 18 times compared with the control group.Morchella mycelium can improve the sucrase and amylase activities in the soil in a short period of time,which is of significance for improving soil.