小檗碱可以通过改善氧化应激和成骨活性减少2型糖尿病大鼠骨量的流失

Berberine can reduce bone mass loss in type 2 diabetic rats by improving oxidative stress and osteogenic activity

目的探索小檗碱对2型糖尿病大鼠骨密度和骨量影响及其可能的机制。方法60只大鼠随机分为4组:对照组(CG)(n=15)、糖尿病组(DG)(n=15)、糖尿病+50 mg/(kg.d)小檗碱治疗组(Brb-50)(n=15)和糖尿病+100 mg/(kg.d)小檗碱治疗组(Brb-100)(n=15)。使用小檗碱治疗12周后,对所有大鼠的股骨进行蛋白印迹、Micro-CT、组织学等评估,并评估其他血液生化指标。结果DG组大鼠的骨密度(BMD)、骨显微结构和骨生物力学指标均明显低于CG组(P<0.05)。氧化指标(8-OHdG)升高,抗氧化剂活性指标SOD、过氧化氢酶、谷胱甘肽过氧化物酶和GST显著降低(P<0.05)。小檗碱治疗后骨密度、骨显微结构、抗氧化剂活性指标均有明显改善。WB结果表明DG组的Runx2和Osterix显著降低;而PPARγ显著上升;小檗碱治疗后Runx2和Osterix明显上升,而PPARγ水平显著降低。结论小檗碱可以通过改善氧化应激和成骨活性减少2型糖尿病大鼠骨量的流失。

Objective To explore the effect of berberine on bone mineral density and bone mass in type 2 diabetic rats and explore the possible mechanism.Methods 60 rats were randomly divided into 4 groups:control group(CG)(n=15),diabetic group(DG)(n=15),diabetic group+50 mg/kg.day berberine treatment(BRB-50)(n=15),and diabetes group+100 mg/kg.day berberine(BRB-100)(n=15).After 12 weeks of treatment with berberine,the femurs of all rats were evaluated by Western blotting,Micro-CT,histology and other blood biochemical indexes.Results The bone mineral density(BMD),bone microstructure and bone biomechanical indexes of rats in DG group were significantly lower than those in CG group(P<0.05).The oxidation index(8-OHdG)increased,while the antioxidant activity indexes SOD,catalase,glutathione peroxidase and GST decreased significantly.After berberine treatment,the bone mineral density,bone microstructure and antioxidant activity were significantly improved.The result of WB showed that Runx2 and Osterix in DG group decreased significantly,while PPARγincreased significantly,Runx2 and Osterix increased,while PPARγlevel decreased after berberine treatment.Conclusion This study shows that berberine can reduce bone mass loss in type 2 diabetic rats by lower oxidative stress and improving osteogenic activity.