金匮肾气丸对去势大鼠骨微结构及ALP、OPG、IL-6的影响

Effects of Jinkui Shenqi pill on bone microstructure and ALP,OPG,IL-6 in ovariectomized rats

目的研究金匮肾气丸防治骨质疏松的机制,探讨“温补肾阳,以生髓壮骨”中医理论在绝经后骨质疏松中的应用。方法采用卵巢切除法复制绝经后骨质疏松大鼠模型,随机分为模型组、金匮肾气丸高、低剂量组、雌二醇组,同时设置假手术组作为对照组,各组分别给予相应的药物灌胃。给药3月后处死取材,计算子宫系数;HE染色观察子宫的病理变化;Micro-CT检测大鼠骨密度及骨微结构的变化;全自动生化分析仪检测各组大鼠血清中血糖、血脂及钙磷的变化;ELISA检测各组大鼠血清中ALP、IL-6及OPG表达的变化。结果与假手术组相比,模型组大鼠骨密度显著下降(P<0.05),骨微结构破坏明显;与模型组相比,金匮肾气丸低剂量组大鼠骨密度得到了有效提高、骨微结构获得改善,血糖、血脂水平降低,血清中钙磷的含量明显提高,血清中ALP、IL-6含量降低,OPG含量升高。结论金匮肾气丸能够提高去卵巢大鼠的骨量,防治绝经后骨质疏松症,其作用机理可能与降低ALP、IL-6,提高OPG含量有关。

Objective To study the mechanism of Jinkui Shenqi Pill in preventing and treating osteoporosis,and to explore the application of TCM theory of“warming kidney-yang and strengthening bones with marrow”in postmenopausal osteoporosis.Methods Postmenopausal osteoporosis rats were induced by ovariectomy.The rats were randomly divided into model group,Jinkui Shenqi pill high dose group,low dose group and estradiol group.At the same time,the sham-operated group served as control group.After 3 months of administration,samples were collected and the uterus coefficient was calculated;pathological changes of uterus were observed by HE staining;changes of bone mineral density and bone microstructure were detected by Micro-CT;changes of blood glucose,blood lipid and calcium and phosphorus in serum were detected by automatic biochemical analyzer;changes of ALP,IL-6 and OPG expression in serum were detected by ELISA.Results Compared with the sham-operated group,the rats in the model group had significantly decreased bone mineral density(P<0.05)and significantly damaged bone microstructure;compared with the model group,the rats in the Jinkui Shenqi pill low dose group had effectively increased bone mineral density,improved bone microstructure,decreased blood glucose and blood lipid levels,significantly increased calcium and phosphorus content in serum,decreased ALP and IL-6 content in serum,and increased OPG content.Conclusion Jinkui Shenqi pill can increase bone mass and prevent postmenopausal osteoporosis in ovariectomized rats,and its mechanism may be related to decreasing ALP,IL-6 and increasing OPG content.