胎盘中信号转导和转录活化因子3与妊娠期肝内胆汁淤积症患者围产结局的关系

Relationship between signal transducer and activator of transcription-3 in placenta and perinatal outcome in patients with intrahepatic cholestasis of pregnancy

目的探讨胎盘中信号转导和转录活化因子3(STAT3)与妊娠期肝内胆汁淤积症(ICP)孕妇分娩结局的关系。方法选择2016年1月至2020年1月我院收治的126例ICP孕妇为观察组,另选择同期入院的102例健康顺产孕妇为对照组。以免疫组织化学链霉素抗生物素蛋白-过氧化物酶链接法(SP法)测定胎盘中STAT3表达,蛋白印迹法测定STAT3蛋白表达。比较两组胎盘中STAT3细胞阳性率、平均光密度值及STAT3蛋白表达,分析胎盘中STAT3表达与ICP孕妇分娩结局的关系。结果观察组胎盘中STAT3细胞阳性率、平均光密度值及STAT3蛋白表达水平显著高于对照组(P均<0.001)。126例ICP孕妇中,顺产占30.95%,剖宫产占69.05%。顺产者胎盘中STAT3细胞阳性率及平均光密度值均显著低于剖宫产者(P均<0.001)。结论胎盘中STAT3表达可能与ICP孕妇分娩结局密切相关。

Objective To investigate the relationship between signal transduction and transcriptional activator 3(STAT3)in placenta and delivery outcome in pregnant women with intrahepatic cholestasis(ICP)during pregnancy.Methods 126 ICP pregnant women admitted to our hospital from January 2016 to January 2020 were selected as the observation group,and 102 healthy pregnant women admitted to our hospital at the same time were selected as the control group.The expression of STAT3 in placenta was determined by immunohistochemical streptomycin(SP)and western blot.The positive rate of STAT3 cells,the average optical density and the expression of STAT3 protein in placenta of the two groups were compared,and the relationship between the expression of STAT3 in placenta and the delivery outcome of ICP pregnant women was analyzed.Results The positive rate,mean optical density and STAT3 protein expression in the placenta of the observation group were significantly higher than that of the control group(all P<0.001).Among the 126 ICP pregnant women,natural delivery accounted for 30.95%and cesarean section for 69.05%.The positive rate and the average optical density of STAT3 cells in the placenta of the parturient were significantly lower than those of the cesarean section(all P<0.001).Conclusion STAT3 in placenta is closely related to the delivery outcome of ICP pregnant women.