黄芩素通过烟酸受体抑制胃癌细胞增殖

The roles of baicalein in proliferation inhibition in gastric cancer cells and its molecular mechanisms

目的:探讨黄芩素抑制胃癌细胞增殖的作用及分子机制。方法:采用CCK-8实验检测不同浓度的黄芩素对胃癌细胞(MGC80-3、HGC-27与BGC-823)增殖作用的影响,以及过表达烟酸受体(GPR109A)对胃癌细胞增殖的影响;Transwell迁移与侵袭实验检测不同浓度的黄芩素对胃癌细胞的体外迁移和侵袭作用的影响;RNA干扰实验检测沉默GPR109A表达对黄芩素抑制胃癌细胞增殖的影响。结果:黄芩素可显著抑制胃癌细胞的体外增殖、迁移与侵袭作用,过表达GPR109A也可显著抑制胃癌细胞的增殖(P<0.05);且沉默GPR109A表达可降低黄芩素对胃癌细胞增殖的抑制作用(P<0.05)。结论:黄芩素可显著抑制胃癌细胞的增殖,且其抑制作用与上调GPR109A的表达密切相关。

Objective: To investigate the function and molecular mechanisms of baicalein in inhibiting the proliferation of gastric cancer cells. Methods: CCK-8 assay was used to detect the baicalein at different concentrations and its effect on the proliferation of gastric cancer cells(MGC80-3, HGC-27, and BGC-823), and the effect of nicotinic acid receptor(GPR109 A) overexpression on the viability of gastric cancer cells. Transwell migration and invasion assay was used to detect the effects of baicalein at different concentrations on the migration and invasion of gastric cancer cells in vitro. RNA interference assay was used to detect the effect of silencing GPR109 A expression on the proliferation of gastric cancer cells inhibited by baicalein. Results: Baicalein significantly inhibited proliferation, migration and invasion of gastric cancer cells in vitro(P<0.05). Overexpression of GPR109 A also markedly inhibited proliferation of gastric cancer cells(P<0.05). Moreover, the expression of GPR109 A silenced by RNAi could block the inhibitory effect of baicalein on the proliferation of gastric cancer cells(P<0.05). Conclusion: Baicalein can significantly suppress the proliferation of gastric cancer cells in vitro. The inhibitory effect of baicalein may be attributed to the upregulation of GPR109 A protein expression in gastric cancer cells.