摘要
目的探讨UBQLN-1(泛素-1)和人Runt相关转录因子3(RUNX3)在直肠癌组织中的表达和临床意义。方法选取2017年6月—2019年6月河北北方学院附属第一医院行手术切除的85例直肠癌组织和相应癌旁正常组织,应用逆转录聚合酶链反应法(RT-PCR)和免疫组化法检测UBQLN-1及RUNX3 mRNA与蛋白水平,分析其与肿瘤临床病理特征间的关系及二者表达的相关性。结果直肠癌组织中UBQLN-1 mRNA表达水平高于癌旁正常组织,RUNX3 mRNA的表达水平低于癌旁正常组织,差异均有高度统计学(P<0.01)。直肠癌组织中UBQLN-1阳性表达率高于癌旁正常组织,RUNX3阳性表达率低于癌旁正常组织,差异有高度统计学意义(P<0.01)。直肠癌组织中UBQLN-1与RUNX3表达呈负相关(r=-0.225,P=0.038)。结论UBQLN-1和RUNX3在直肠癌组织中的异常表达与其发生发展、演进密切相关,且二者呈明显负相关,可作为直肠癌诊断、治疗和预后评估的潜在分子生物学指标。
Objective To explore the expression of UBQLN-1 and RUNX3 in rectal cancer tissues and clinical significance.Methods From June 2017 to June 2019,85 cases of rectal cancer tissues and adjacent normal tissues were selected from the First Affiliated Hospital of Hebei North University were selected.The mRNA and protein levels of UBQLN-1 and RUNX3 were detected by reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry and the relationship between UBQLN-1,RUNX3 and the clinicopathological characteristics of tumors and the correlation between their expressions were analyzed.Results The expression of UBQLN-1 mRNA in rectal cancer tissues was higher than that in adjacent normal tissues;and the expression of RUNX3 mRNA in rectal cancer tissues was lower than that in adjacent normal tissues,and the differences were highly statistically significant(P<0.01).The positive expression rate of UBQLN-1 in rectal cancer tissues was higher than that in adjacent normal tissues and the positive expression rate of RUNX3 was lower than that of adjacent normal tissues,with highly statistically significant differences(P<0.01).UBQLN-1 was negatively correlated with RUNX3 expression in rectal cancer tissues(r=-0.225,P=0.038).Conclusion The abnormal expressions of UBQLN-1 and RUNX3 in the rectal cancer tissues are closely related to their development and evolution,and rectal cancer tissues and adjacent normal tissues are significantly negatively correlated,which can be used as potential molecular biological indicators for the diagnosis,treatment and prognosis assessment of rectal cancer.
作者
孙光源
郭圣超
武雪亮
薛军
屈明
郭飞
韩磊
韩洁
SUN Guangyuan;GUO Shengchao;WU Xueliang;XUE Jun;QU Ming;GUO Fei;HAN Lei;HAN Jie(Department of General Surgery,the First Affiliated Hospital of Hebei North University,Hebei Province,Zhangjiakou075061,China;Graduate School,Hebei North University,Hebei Province,Zhangjiakou075061,China;Department of Ophtalmology,the Fourth Hospital of Zhangjiakou,Hebei Province,Zhangjiakou075061,China)
出处
《中国医药导报》
CAS
2020年第27期28-32,F0003,共6页
China Medical Herald
基金
河北省科技计划项目(152777237)
河北省医学科学研究课题计划项目(20190913)。
关键词
泛素-1
人Runt相关转录因子3
直肠癌
临床意义
Ubiquitin-1
Human RUNt-related transcription factor 3
Rectal cancer
Clinical significance