外周血淋巴细胞亚群比例紊乱与帕金森病的相关性研究

Correlation between the disorder of peripheral blood lymphocyte subsets ratio and Parkinson′s disease

目的通过检测帕金森病(PD)患者外周血中各淋巴细胞亚型的百分率差异,探讨神经免疫机制与PD发生的相关性。方法应用荧光染色法测定新疆医科大学第一附属医院(以下简称“我院”)2014年6月—2017年7月收治的原发性PD患者(病例组,83例)及同期我院健康体检者(对照组,100名)外周血中CD3^+、CD4^+、CD8^+、CD19^+、CD56^+、Fas、Tregs细胞百分率差异,并进一步分析病例组患者是否服用药物复方左旋多巴、Hoehn-Yahr(HY)分级差异与各淋巴细胞亚群变化相关性。结果病例组CD3^+、CD4^+、CD8^+低于对照组,CD56^+细胞、Fas和Tregs阳性亚群高于对照组,差异有高度统计学意义(P<0.01)。服药组Fas和Tregs细胞亚群低于未服用组,差异有统计学意义(P<0.05)。相关性分析显示,Fas和Tregs阳性细胞数与HY分级呈正相关(r=0.620、0.761,P<0.05)。结论PD患者外周血各淋巴细胞亚群存在比例紊乱,其中Fas及Tregs细胞比例与PD患者病情严重程度有关,对于未口服复方左旋多巴治疗的PD患者该比例会进一步升高。

Objective To explore the correlation between neural immune mechanisms and the occurrence of Parkinson′s disease(PD)by detecting the percentage differences of lymphocyte subtypes in peripheral blood of patients with PD.Methods Fluorescence staining method was used to determine the periphery of primary PD patients(case group,83 cases)admitted to the First Affiliated Hospital of Xinjiang Medical University(“our hospital”for short)from June 2014 to July 2017 and healthy physical examination patients(control group,100 cases)in our hospital during the same period,while the differences in the percentage of CD3^+,CD4^+,CD8^+,CD19^+,CD56^+,Fas and Tregs cells in the blood were measured,and the correlation between whether the patients in the case group took the drug Compound Levodopa,Hoehn-Yahr(HY)grade difference and the changes of lymphocyte subsets were analyzed.Results CD3^+,CD4^+,and CD8^+in the case group were lower than those in the control group,while the positive subsets of CD56^+cells,Fas and Tregs were higher than those in the control group,and the differences were highly statistically significant(P<0.01).Fas and Tregs cell subsets in the medication group were lower than those in the non-administration group,and the differences were statistically significant(P<0.05).Correlation analysis showed that the number of Fas and Tregs positive cells were positively correlated with HY grade(r=0.620,0.761,P<0.05).Conclusion Peripheral blood lymphocyte subsets of PD patients have a disorder in the proportions.The the proportion of Fas and Tregs cells are related to the severity of the disease in PD patients.For PD patients who are not treated with oral Compound Levodopa,the proportion will further increase.