益气化瘀散结方对胃癌SGC-7901细胞PI3K/AKt/Mtor信号通路的影响

Effects of Yiqi Huayu Sanjie Decoction on PI3K-AKT-mTOR Signal Pathway of Gastric Cancer Cell Line of SGC-7901

目的:通过观察益气化瘀散结方干预后胃癌SGC-7901细胞PI3K/AKt/mTOR信号通路分子的表达变化,探讨益气化瘀散结方影响胃癌细胞增殖的作用机制。方法:体外培养SGC-7901细胞,采用益气化瘀散结方含药血清干预,细胞分为空白血清组及5%、10%、20%益气化瘀散结方含药血清组,药物干预后,MTT法检测细胞增殖情况,流式细胞术检测细胞周期变化,Western-blot、Real-time PCR技术检测PI3K、AKT、mTOR蛋白及mRNA的表达变化。结果:与空白血清组比较,不同浓度益气化瘀散结方含药血清均能不同程度抑制SGC-7901细胞的增殖,其中10%,20%更为明显,差异有统计学意义(P<0.05),时间上以48 h最为明显;与空白血清组比较,10%,20%益气化瘀散结方含药血清组G0/G1期细胞比例明显升高,S期细胞比例明显降低(P<0.05);与空白血清组比较,不同浓度益气化瘀散结方含药血清组PI3K、AKT、mTOR蛋白及mRNA表达水平明显降低,差异有统计学意义(P<0.05)。结论:抑制PI3K/AKt/mTOR信号通路可能是益气化瘀散结方抑制胃癌细胞增殖、调节胃癌细胞生长周期的重要作用机制之一。

Objective:To observe the expression changes of PI3K/AKt/mTOR signaling pathway molecule of gastric cancer cell line of SGC-7901,and to explore the mechanism of Yiqi Huayu Sanjie Decoction on affecting the proliferation of gastric cancer cells.Methods:SGC-7901 cells were cultured in vitro and treated with drug-containing serum of Yiqi Huayu Sanjie Decoction.Cells were divided into a blank serum group,5%,10%,20%of drug-containing serum of Yiqi Huayu Sanjie Decoction groups.After drug intervention,the cell proliferation was detected by MTT,the cell cycle change was detected by flow cytometry,the protein and mRNA expression of PI3K,AKT and mTOR were detected by Western-blot and Real-time PCR.Results:Compared with the blank serum group,different concentration of Yiqi Huayu Sanjie Decoction could inhibit the proliferation of SGC-7901 cells to some degree,in which 10%and 20%concentration were especially significant and the difference was statistically significant(P<0.05).The most obvious time was 48 h;compared with the blank serum group,the proportion of cells in the G0/G1 phase increased and S phase decreased significantly in 10%and 20%concentration of Yiqi Huayu Sanjie Decoction group drug-containing serum group(P<0.05);compared with the blank serum group,the expression of PI3K,AKT,mTOR protein and mRNA in serum of different concentration of Yiqi Huayu Sanjie Decoction were significantly decreased,the difference was statistically significant(P<0.05).Conclusion:Inhibition of PI3K/AKt/mTOR signaling pathway may be one of the important mechanisms of Yiqi Huayu Sanjie Decoction on inhibiting the proliferation and regulating the growth cycle of gastric cancer cells.