马铃薯StUOXs基因家族的生物信息学分析

Bioinformatics analysis of StUOXs gene family in potato

马铃薯(Solanum tuberosum)是世界第三大粮食作物,其产量和品质受到生物与非生物胁迫的影响。泛醌氧化酶(ubiquinol oxidase,UOX)是一种由核基因编码的线粒体末端氧化酶,在植物生长发育及胁迫应答中发挥重要作用。为了解StUOXs在马铃薯抗病过程中发挥的应答反应,利用生物信息学方法在马铃薯基因组中鉴定出5个StUOXs基因,对其理化性质、染色体定位、三级结构、进化关系、保守结构域、启动子元件和表达谱尤其是受青枯菌(Ralstonia solanacearum)诱导的表达模式进行了分析。结果表明:马铃薯StUOXs基因编码的氨基酸数在279~366,相对分子质量为31~42 ku,亚细胞定位在线粒体中;系统进化分析发现,马铃薯StUOXs成员与番茄、辣椒的UOXs成员的亲缘关系最近,较之拟南芥、烟草亲缘关系稍远;FPKM数据分析发现,StUOXs主要受盐、激素和生物胁迫诱导而在叶片中表达,这与其启动子中的存在的激素应答等顺式作用元件相对应。青枯菌接种后的qRT-PCR实验表明,StUOX4参与马铃薯青枯病的早期应答反应。

Potato(Solanum tuberosum)is the third largest food crop in the world,and its yield and quality are adversely affected by biotic and abiotic stresses.Ubiquinone oxidase(UOX),as a mitochondrial terminal oxidase encoded by nuclear genes,plays an important role in the regulation of plant growth and stress response.In order to understand the response of StUOXs in potato disease resistance,five StUOXs genes were identified in potato genome by bioinformatics methods,and their physical and chemical properties,chromosome location,tertiary structure,evolutionary relationship,conserved domain,promoter element and expression profile,especially the expression patterns induced by Ralstonia solanacearum were analyzed.The results showed that the number of amino acids encoded by potato StUOXs genes was between 279 and 366,the molecular weight was distributed between 31 and 42 ku,and the subcellular locations were located in mitochondria.Phylogenetic analysis showed that potato StUOXs members were closely related to tomato and pepper UOXs members,but slightly farther than Arabidopsis thaliana and tobacco.FPKM data analysis showed that StUOXs were mainly induced by salt,hormone and biological stress in leaves,which corresponded to the cis-acting elements such as hormone response in its promoter.The qRT-PCR test after inoculation with Ralstonia solanacearum showed that StUOX4 was involved in the early response to the bacterial wilt pathogens.