氨氮降解微生物的筛选和初步应用

Screening and utilization of ammonia-nitrogen-degrading microorganism

为了创制高效的粪污氨氮降解菌剂,特从猪粪中分离高效的氨氮降解微生物。通过感官法初筛,并结合氨氮降解率为指标进行复筛,最终获得高效氨氮降解菌株——Z2、AOZ和Y。经形态学观察、生理生化鉴定、16S rRNA基因序列分析,确定菌株Z2为弯曲芽孢杆菌(Bacillus flexus),菌株AOZ为恶臭假单胞菌(Pseudomonas putida),Y为干酪乳杆菌(Lactobacillus casei)。试验结果显示,菌株Z2和Y组成的复配菌剂对氨氮的降解效果最优,氨氮降解率高达53.19%,表明该复合菌剂可以应用于粪污除氨。

To obtain the ammonia-lowering agent for livestock manure,the bacterial strains with good ammonia-nitrogen-degrading capability were isolated from swine manure.Three strains with high ammonia-nitrogen degradation efficiency were identified with the olfactory threshold method combined with the comparisons of their ammonia-nitrogen degradation efficiencies,and were encoded as Z2,AOZ and Y,respectively.Based on the results of morphological observation,physiological and biochemical tests,and 16S rRNA sequence homology analysis,the strains Z2,AOZ and Y were identified as Bacillus flexus,Pseudomonas putida,and Lactobacillus casei,respectively.The experimental results showed that the ammonia-nitrogen degradation efficiency was higher when strains Z2 and Y were mixed,and the highest ammonia-nitrogen degradation efficiency reached 53.19%,suggesting that the microorganism complex could be practically applied to remove ammonium from swine manure.