一个基于菲咯啉的Pb(Ⅱ)荧光探针

A Novel Phenanthroline-Based Fluorescent Probe for Pb(Ⅱ)

设计并合成了一种基于菲咯啉的Pb^2+荧光探针L.在探针L的DMF-H2O(V:V=1:1,10 mmol/L Tris,pH=7.4)溶液中,加入Pb2+后导致其荧光猝灭,同时可以观察到溶液的颜色由黄色变为橙色,检测限低至2.68×10^-7 mol/L.通过紫外光谱、荧光光谱、高分辨质谱、核磁共振氢谱和计算拟合等手段研究了传感器L和Pb^2+离子的络合模式.进一步研究发现,L可以作为一种重复利用的检测Pb^2+荧光探针,并用于对活细胞中的Pb^2+的荧光成像.

A novel fluorescent probe L for Pb^2+based phenanthroline derivative has been designed and synthesized.The probe L displayed fluorescence quenching emission excellent sensitivity specific toward Pb^2+ion.The introduction of Pb^2+ resulted the quenching fluorescence of probe L and the color of the solution can be observed changes from yellow to orange in DMF-H2O(V:V=1:1,10 mmol/L Tris,pH=7.4).The detection limit was as low as 2.68×10^-7 mol/L.The bonding mechanism was confirmed by UV-vis,fluorescence,HRMS,1H NMR experiments and theoretical calculation.It was found that the probe had excellent reversibility for detection of Pb^2+ and could be used to effectively identify Pb^2+ in living cells.