布鲁菌病患者血清sTim-3、Galectin-9水平与炎症因子IFN-γ、IL-4水平的相关性

Correlation between serum levels of sTim-3,Galectin-9 and inflammatory factors IFN-γ,IL-4 in patients with brucellosis

目的探讨布鲁菌病患者血清可溶性T细胞免疫球蛋白黏蛋白分子3(sTim-3)、半乳糖凝集素-9(Galectin-9)水平与炎症因子干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平的相关性。方法选择2016年1月至2018年12月烟台业达医院收治的布鲁菌病患者90例为研究对象纳入观察组,其中急性期49例、慢性期41例;另选择同期进行健康体检的健康受试者50例为对照组。采集布鲁菌病患者和健康受试者空腹静脉血5 ml,采用酶联免疫吸附实验(ELISA)法检测sTim-3、Galectin-9水平,流式液相多重蛋白定量技术(CBA)检测IFN-γ、IL-4水平;并应用Pearson相关性检验对布鲁菌病患者血清中的sTim-3、Galectin-9及sTim-3/Galectin-9水平与IFN-γ、IL-4水平进行相关性分析。结果观察组、对照组人群血清中sTim-3[(18.28±5.38)、(7.01±1.97)ng/L]、Galectin-9[(2.79±0.94)、(1.67±0.23)ng/L]、sTim-3/Galectin-9(6.55±2.96、4.20±0.67)、IFN-γ[(5.99±1.94)、(10.09±2.03)ng/L]、IL-4[(7.34±3.13)、(3.71±1.76)ng/L]水平组间比较差异有统计学意义(t=14.271、8.276、5.527、11.785、7.556,P均<0.05)。观察组中,急性期、慢性期布鲁菌病患者sTim-3[(22.09±3.13)、(14.21±3.42)ng/L]、Galectin-9[(2.98±0.67)、(2.41±0.74)ng/L]、sTim-3/Galectin-9(7.41±1.07、5.90±1.72)、IFN-γ[(3.78±1.65)、(7.02±1.73)ng/L]、IL-4[(9.17±2.04)、(5.67±1.54)ng/L]水平组间比较差异有统计学意义(t=11.402、3.833、5.084、9.075、9.037,P均<0.05)。经Pearson相关性检验,布鲁菌病患者的sTim-3、Galectin-9、sTim-3/Galectin-9水平与IFN-γ水平呈负相关(r=-0.568、-0.531、-0.519,P均<0.05),与IL-4水平呈正相关(r=0.517、0.524、0.536,P均<0.05)。结论布鲁菌病患者血清中sTim-3、Galectin-9呈现高表达,且与IFN-γ、IL-4水平显著相关,提示sTim-3、Galectin-9可能与布鲁菌病的发生、发展过程有关。

Objective To explore the correlation between serum soluble T cell immunoglobulin-mucin molecule-3(sTim-3),galactose lectin-9(Galectin-9)and inflammatory factors interferon-γ(IFN-γ),interleukin-4(IL-4)in patients with brucellosis.Methods Ninety patients with brucellosis admitted to Yantai Yeda Hospital from January 2016 to December 2018 were selected as the observation group,of whom 49 cases in acute stage and 41 cases in chronic stage.Another 50 healthy subjects who underwent health examination during the same period were selected as the control group.Five milliliter of fasting venous blood samples were collected from patients with brucellosis and healthy subjects,and enzyme-linked immunosorbent assay(ELISA)was used to detect sTim-3 and Galectin-9 levels,and cytometric bead array(CBA)to detect IFN-γand IL-4 levels;Pearson correlation test was used to correlate serum levels of sTim-3,Galectin-9,sTim-3/Galectin-9 and inflammatory factors IFN-γ,IL-4 in patients with brucellosis.Results The levels of sTim-3[(18.28±5.38),(7.01±1.97)ng/L],Galectin-9[(2.79±0.94),(1.67±0.23)ng/L],sTim-3/Galectin-9(6.55±2.96,4.20±0.67),IFN-γ[(5.99±1.94),(10.09±2.03)ng/L],IL-4[(7.34±3.13),(3.71±1.76)ng/L]were significantly different between the observation group and control group(t=14.271,8.276,5.527,11.785,7.556,P<0.05).In the observation group,the levels of sTim-3[(22.09±3.13),(14.21±3.42)ng/L],Galectin-9[(2.98±0.67),(2.41±0.74)ng/L],sTim-3/Galectin-9(7.41±1.07,5.90±1.72),IFN-γ[(3.78±1.65),(7.02±1.73)ng/L],and IL-4[(9.17±2.04),(5.67±1.54)ng/L]were statistically significant difference between patients with acute and chronic brucellosis(t=11.402,3.833,5.084,9.075,9.037,P<0.05).Pearson correlation test showed that the levels of sTim-3,Galectin-9,and sTim-3/Galectin-9 in patients with brucellosis were negatively correlated with IFN-γlevel(r=-0.568,-0.531,-0.519,P<0.05),and positively correlated with IL-4 level(r=0.517,0.524,0.536,P<0.05).Conclusion The expressions of sTim-3 and Galectin-9 in serum of brucellosis patients are high,and they are significantly correlated with IFN-γand IL-4 levels,suggesting that sTim-3 and Galectin-9 maybe related to the occurrence and development of brucellosis.