体细胞克隆技术在乌金猪遗传资源保护上的应用

Application of Somatic Cell Cloning Technology in the Protection of Genetic Resources of Wujin Pigs

旨在通过猪体细胞克隆技术,生产乌金猪火毛系,并分析生长发育能力及繁殖性能,为该技术在地方优良猪种保种上的应用提供理论依据。本研究采集了符合乌金猪种质特征的3头公猪和12头母猪的耳组织样品。公猪年龄分别为3、6、11月龄,母猪年龄差距较大,最小的2月龄,最大的10岁。其中3头母猪和1头3月龄公猪已被去势,10岁母猪已无繁殖能力。结果,成功建立了15头乌金猪的耳组织成纤维细胞系,分别选择1头3月龄乌金猪(♂)和10岁乌金猪(♀)的成纤维细胞进行体细胞核移植,经胚胎移植入16头代孕母猪,共获得25头克隆猪,克隆猪生长发育正常,性成熟后进行自然交配共获得39头F1代活仔。本研究通过体细胞克隆技术成功克隆了乌金猪,具备正常的生长发育性能和繁殖性能,为地方猪种的保护研究提供了新的途径。

The purpose of this experiment was to study the growth and developmental ability and reproductive performance of cloned Wujin pigs fire hair line by somatic cell cloning technology,and provide a theoretical basis for the application of somatic cell cloning technology in the conservation of local excellent pig breeds.In this study,the ear samples were collected from 3 male and 12 female pigs which met the characteristics of Wujin pigs.The 3 boars were 3,6 and 11 months old,respectively.The sows had a large age gap,from 2 months old to 10 years old,the three sows and one boar of 3-month-old had been castrated and the sow of 10-year-old had no reproductive capacity.The ear fibroblast cell lines were successfully established from 15 Wujin pigs.Then the fibroblast cells from 3-month-old(♂)and 10-year-old(♀)pigs were used as donor cells for somatic cell nuclear transfer.The cloned embryos were transplanted into 16 surrogate sows and 25 cloned pigs were obtained.The growth and development indexes of these cloned pigs were all normal.After sexual maturity,the male and female cloned pigs mated naturally and 39 live piglets(F1)were obtained.This study successfully cloned Wujin pigs by somatic cell nuclear transfer technology,and cloned Wujin pigs had normal growth and developmental ability and reproductive performance.The study result provides a new way for the conservation of local pig breeds.