猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析

Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis

通过比较猪囊等孢球虫孢子化卵囊与未孢子化卵囊的差异表达蛋白质,以期从蛋白质组学角度研究其发育机制,并为此类球虫的发育机制及该病的免疫预防或化学防治找到新的“关键”分子奠定基础。首先建立哺乳仔猪的猪囊等孢球虫感染模型,然后运用iTRAQ技术,结合LC-MS/MS分析差异表达蛋白质,并采用荧光定量PCR验证iTRAQ数据。结果共鉴定出174个蛋白,差异表达蛋白40个,其中38个蛋白上调表达,2个蛋白下调表达,差异蛋白主要涉及代谢、抗生素的生物合成、次生代谢产物的生物合成和糖酵解/糖异生过程等通路。研究结果为阐明猪囊等孢球虫孢子化发育机制以及发现新的生物标志物提供参考。

The differentially expressed proteins between sporulated oocyst and unsporulated oocyst of Cystoisospora suis strains were screened and compared.In this study,the C.suis infection model of piglets were established and a quantitative proteomic analysis between sporulated oocyst and unsporulated oocyst of C.suis were conducted by iTRAQ labeling and LC-MS/MS.Part of the iTRAQ results were validated by qRT-PCR.Total 174 proteins were identified,among which 40 proteins were differentially expressed,38 proteins were upregulated and 2 proteins were downregulated.The differentially expressed proteins were mainly involved in metabolism,antibiotics biosynthesis,secondary metabolites biosynthesis and glycolysis/gluconeogenesis pathways.The results of this study provide reference for elucidating the developmental mechanism of C.suis sporulated oocyst and facilitated discovering a new biomarker for C.suis.