荔波产香茶菜化学成分研究

Chemical constituents from Isodon amethystoides distributed in Libo

目的研究荔波产香茶菜Isodon amethystoides的化学成分。方法选用硅胶、MCI gel CHP 20P、Sephadex LH-20凝胶柱色谱等方法进行分离和纯化,通过波谱数据、理化性质结合参考文献鉴定化合物结构。结果从荔波产香茶菜甲醇提取物中分离得到了18个化合物,分别鉴定为线纹香茶菜酸(1)、雪花草丁素(2)、4,9α-环氧-3,4-开环异海松烷-7,15-二烯-3-酸(3)、3,4-开环异海松烷-4(18),7,15-三烯-3-酸甲酯(4)、3,4-开环异海松烷-4(18),7,15-三烯-3-酸(5)、异海松烷-7,15-二烯-3-酮(6)、异海松烷-7,15-二烯-3β-醇(7)、山姜萜醇(8)、(-)-丁香三环烷-2,9-二醇(9)、乌苏酸(10)、齐墩果酸(11)、3-表-马斯里酸(12)、7α-羟基谷甾醇(13)、β-谷甾醇(14)、豆甾醇(15)、芝麻素(16)、泡桐素乙酯(17)、邻苯二甲酸二丁酯(18)。结论化合物4为首次分离得到的天然产物,2~9、15~18为首次从香茶菜中分离到。

Objective To investigate the chemical constituents from Isodon amethystoides distributed in Libo. Methods Chemical constituents were isolated and purified by chromatography with silica gel, MCI gel CHP 20P, and Sephadex LH-20. The structures of all compounds were elucidated by physicochemical properties, comprehensive spectral data and references. Results Eighteen known compounds were isolated from the methanol extract of I. amethystoides, which were elucidated as lophanic acid(1), rubesanolide D(2), fladin A(3), 3,4-secoisopimara-4(18),7,15-triene-3-oic acid methylester(4), 3,4-secoisopimara-4(18),7, 15-triene-3-oic acid(5), isopimara-7,15-dien-3-one(6), isopimara-7,15-dien-3β-ol(7), alpiniol(8),(-)-clovane-2,9-diol(9), ursolic acid(10), oleanolic acid(11), epimaslinic acid(12), 7α-hydroxysitosterol(13), β-sitosterol(14), stigmasterol(15), sesamin(16), paulownin acetate(17) and dibutyl phthalate(18). Conclusion Compound 4 is a new natural product, and compounds 2—9, 15—18 are isolated from I. amethystoides for the first time.