不同重楼变种间内生真菌群落多样性差异及生物标记物初探

Diversity and biomarker exploration of endophytic fungi communities in different varieties of Paridis Rhizoma

目的通过对不同变种重楼内生真菌群落多样性研究及生物标记物分析,为中药材重楼多基源质量等同性及鉴别溯源技术的开发研究奠定基础。方法采用Illmina Hiseq高通量测序技术对3个变种的重楼内生真菌群落基因进行扩增子序列测定,同时对菌群多样性、真菌群落差异进行生物信息学分析。结果对不同变种重楼内生真菌进行多样性分析结果显示,七叶一枝花Paris polyphylla Smith var. chinensis (Franch.) Hara、长药隔重楼Paris polyphylla Sm. var. thibetica (Franch.) Hara、具柄重楼Paris fargesii Franch. var. petiolate (Baker ex C. H. Wright) Wang et Tang三者的内生真菌的α多样性存在差异,但β多样性无显著性差异,具柄重楼中特有的OTU仅为0.27%。真菌群落注释结果表明,3个变种分别注释到6个相同的门,但组成比率不同。另外,通过LEfSe分析,初步探索了3个变种的生物标记物差异,七叶一枝花中Rhizophagus irregularis与其他2变种有显著性差异。结论 3个变种重楼内生真菌群落β多样性变化不显著,但组成比率有很大的差异性。该研究结果中药材重楼多基源质量等同性及鉴别溯源技术的开发研究提供研究基础。

Objective The analysis of endophytic fungi diversity and the biomarkers in different varieties of Paridis Rhizoma were used to lay a foundation for the development of quality equivalence and identification and traceability technology. Methods The amplicons of endophytic fungal community genes from three varieties of Paridis Rhizomaa were sequenced by using Illmina Hiseq high-throughput sequencing. Bioinformatic analysis was performed to study the diversity of flora and the difference of fungal community. Results The α diversity of endophytic fungi from different varieties was different, but there was no significant difference in β diversity. The OTU of Paris fargesii var. petiolata(Baker ex C. H. Wright) in endophytic fungi was only 0.27%. The results of fungal community annotation showed that the three varieties were annotated to six identical phyla, but the composition ratios were different. Moreover, we explored the biomarker differences in three variants, and the Rhizophagus irregularis of Paris polyphylla through LEfSe analysis. is a biomarker with significant difference. Conclusion The diversity change of endophytic fungi community of the three varieties was not significant, but the composition ratio was different. The results of this study provided the research basis for the development of quality equivalence and identification and traceability technology.