Anti-migratory effects of Piper betle leaf aqueous extract on cancer cells and its microtubule targeting properties

槟榔叶水提液对癌细胞的抗迁移作用及其微管靶向性的研究

Piper betle(PB),also known as ubeter,in Malay language,is a tropical Asian vine.PB leaves are commonly chewed by Asians along with betel quid.It contains phenols such as eugenol and hydroxychavicol along with chlorophyll,P-carotene,and vitamin C(Salehi et al.,2019).Extracts from PB leaves have various medicinal properties including anticancer,antioxidant,anti-inflammatory,and antibacterial effects(Salehi et al.,2019).Previous research has shown that PB induces cell cycle arrest at late S or G2/M phase and causes apoptosis at higher doses(Wu et al.,2014;Guha Majumdar and Subramanian,2019).

本文研究了槟榔(Piperbetle,PB)水提取物对癌细胞转移能力的调节作用及其对微管蛋白结构和网络的影响。采用亚毒剂量对PB和5-氟尿嘧啶(5-FU)处理的癌细胞进行抗迁移研究,观察细胞迁移20小时。在所有处理组中,100μg/m L PB处理的癌细胞均显示出最大的抗迁移作用(P=0.016)。总体上,PB对癌细胞的抗迁移作用高于5-FU。通过细胞形态观察发现,PB处理的细胞表现出与标准微管抑制剂(紫杉醇)相似的细胞特征,并在PB和紫杉醇处理的癌细胞中发现M期细胞群。通过对微管结构和网络进一步研究,我们发现PB和紫杉醇处理的癌细胞表现出长期破坏的纺锤体。因此,我们认为PB具有对癌细胞的抗迁移作用,并可能改变蛋白结构和网络。