某口服液对高尿酸血症大鼠模型的影响

Effects of an oral liquid on hyperuricemia rat model

目的探讨某口服液对高尿酸血症大鼠模型的作用。方法取SD大鼠90只,按血尿酸水平随机分成6组,每组15只,分别为正常对照组、模型对照组、阳性对照组[别嘌醇27.0 mg/(kg·bw)]、受试口服液低、中、高3个剂量组[1.67、3.33、10.00 ml/(kg·bw)],除正常对照组外,其余各组动物采用腺嘌呤+氧嗪酸钾诱导高尿酸血症,同时给予各受试液连续灌胃30 d,测定血清中尿酸、肌酐、尿素氮水平。取肾进行病理切片。结果与模型对照组比较,受试口服液低、中、高剂量组实验末期血清尿酸和肌酐水平降低,差异有统计学意义(P<0.01);肾脏病理结果显示,与模型对照组比较,受试口服液中、高剂量组肾小管病变程度缓解。结论受试口服液对大鼠高尿酸血症具有明显的改善作用。

Objective To explore the effects of an oral liquid on hyperuricemia rat model.Methods According to the level of serum uric acid,90 SD rats were randomly divided into 6 groups(each n=15),including the normal control group,the model control group,the positive control group(allopurinol 27.0 mg/kg.bw)and low-,medium-and high-dose groups of uric acidlowering oral solution(1.67 ml/kg.bw,3.33 ml/kg.bw,10.00 ml/kg.bw).Rats of all groups received adenine+potassium oxonate compound to induce hyperuricemia except the normal control group,and then test solution was given to the rats by oral gavage for 30 successive days.Serum uric acid(UA),creatinine(Cr)and urea nitrogen(BUN)were measured.The kidneys were taken for pathological sectioning examination.Results At the end of the experiment,serum levels of UA and Cr were significantly lower in low-,medium-and high-dose groups of uric acid-lowering oral solution than in the model control group(all P<0.01).Renal pathological results showed that the degree of renal tubular lesions was significantly relieved in the middle-and high-dose groups of uric acid-lowering oral solution as compared with the model control group.Conclusions The uric acidlowering oral liquid can significantly improve the rats’hyperuricemia induced by adenine+potassium oxonate compound.