蒸发光散射液相色谱法测定保健食品中磷脂酰胆碱的含量

Determination of phosphatidylcholine in health food by high performance liquid chromatography with evaporative light scattering detector

目的采用蒸发光散射检测器,建立高效液相色谱法测定保健食品中磷脂酰胆碱的含量。方法以正已烷-异丙醇水溶液(0.1%的三乙胺,三氟乙酸调pH至3.5)为流动相,采用正相硅胶色谱柱和蒸发光散射检测器,高效液相色谱仪测定保健食品中磷脂酰胆碱的含量。结果在0.05~2.0 mg/ml范围内,蒸发光散射检测器响应值的对数与磷脂酰胆碱质量浓度的对数线性相关,检测限为0.02 mg/ml。样品加标回收率为93.7%~102.3%,精密度(n=7)在1.3%以下。结论液相色谱蒸发光散射法能够快速测定保健食品中磷脂酰胆碱的含量,具有操作简便、准确度高和重现性好等优点,适用于保健食品中磷脂酰胆碱含量的测定。

Objective To establish a method for determination of phosphatidylcholine in health food by high performance liquid chromatography with evaporative light scattering detector(HPLC-ELSD).Methods Using n-hexane-isopropanol aqueous solution(0.1%triethylamine and pH 3.5 adjusted by trifluoroacetic acid)as mobile phase,phosphatidylcholine in health food samples was determined by HPLC-ELSD equipped with a silica gel column.Results In the range of 0.05 mg/ml-2.0 mg/ml,the logarithm of the response value of ELSD was linearly related to the logarithm of the concentration of phosphatidylcholine,and the detection limits were 0.02 mg/L.The recovery rates were 93.7%-102.3%,and the precision(n=7)was less than 1.3%.Conclusions The developed HPLC-ELSD method can rapidly determine the content of phosphatidylcholine in health food with the advantages of simple operation,high accuracy and good reproducibility,and it is suitable for determination of phosphatidylcholine content in health food.