女性尿液沙眼衣原体DNA和淋病奈瑟菌DNA定性检测结果分析

Quantitative analysis of DNA of Chlamydia trachomatis and Neisseria gonorrhoeae of female urine

目的探讨用女性尿液检测沙眼衣原体(chlamydia trachomatis,CT)DNA和淋病奈瑟菌(neisseria gonorrhoeae,NG)DNA的临床意义。方法随机选取2019年1—6月性病科女性患者212例,每位患者取宫颈拭子做CT-DNA、NG-DNA、淋球菌培养,尿液做CT-DNA、NG-DNA。同时设置对照组30例。结果宫颈拭子CT-DNA阳性率为18.40%(39/212);尿液CT-DNA阳性率为19.81%(42/212),二者阳性率比较差异无统计学意义(χ2=0.14,P=0.71);宫颈拭子CT-DNA和尿液CT-DNA检测结果一致性好(Kappa=0.92,P<0.01)。淋球菌培养阳性率为5.19%(11/212),宫颈拭子NG-DNA阳性率为12.26%(26/212),二者阳性率比较差异有统计学意义(χ2=6.662,P=0.010);尿液NG-DNA阳性率为10.38%(22/212),与淋球菌培养阳性率相比,差异有统计学意义(χ2=3.976,P=0.046);宫颈拭子NG-DNA阳性率与尿液NG-DNA阳性率相比,差异无统计学意义(χ2=0.376,P=0.540)。宫颈拭子NGDNA与尿液NG-DNA检测结果一致性好(Kappa=0.86,P<0.01)。结论用女性尿液检测CT-DNA、NG-DNA敏感性高、特异性好,取材方便,建议在临床普及。

Objective To investigate the clinical significance of detecting Chlamydia trachomatis(CT)DNA and Neisseria gonorrhoeae(NG)DNA in female urine.Methods From January to June 2019,212 female patients in department of venereal disease,were randomly selected.Cervical swabs were taken from each patient to detect CT-DNA,NG-DNA,Neisseria gonorrhoeae culture,and urine was used to detect CT-DNA,NG-DNA.Thirty female examinees were chosen as the control group.Results CT-DNA positive rate of cervical swabs was 18.40%(39/212);CT-DNA positive rate of urine was 19.81%(42/212);there was no statistically significant difference between the positive rates of the two(χ2=0.14,P=0.71).The results of cervical swab CT-DNA and urine CT-DNA were consistent(Kappa=0.92,P<0.01).The positive rate of N.gonorrhoeae culture was 5.19%(11/212),and the positive rate of NG-DNA in cervical swabs was 12.26%(26/212),there was statistically significant difference between the positive rates of the two(χ2=6.662,P=0.010);the NG-DNA positive rate of urine was 10.38%(22/212),there was statistically significant difference compared with the positive rate of N.gonorrhoeae culture(χ2=3.976,P=0.046);the NG-DNA positive rate of cervical swabs was similar to that of urine(χ2=0.376,P=0.540).The results of cervical swab NG-DNA and urine NG-DNA were consistent(Kappa=0.86,P<0.01).Conclusion Urine is a non-invasive specimen.The detection of CT-DNA and NG-DNA of urine in female patients has high sensitivity and specificity,suggested for clinical popularity.