白细胞介素-1基因多态性及幽门螺旋杆菌感染与健康中年人群动脉粥样硬化程度的相关性

The relationship between the interleukin-1 gene polymorphism and Helicobacter pylori infection,and atherosclerosis in healthy middle-aged population

目的:探讨白细胞介素(IL)-1基因IL-1β-511、IL-1 RN VNTR位点多态性在幽门螺旋杆菌(HP)感染加重健康中年人群动脉粥样硬化程度中的作用。方法:以聚合酶链反应限制性片段长度多态性分析法检测227名健康中年人IL-1β-511、IL-1 RN VNTR位点多态性,ELISA法检测HP IgG及CagA、VacA抗体,通过臂踝指数(ABI)评估受试者动脉粥样硬化程度。结果:IL-1β-511多态性分别为CT(64.8%)、TT(22.3%),IL-1RN VNTR片段倍数多态性分别为IL-1RN*1(68.7%)、2倍重复(IL-1RN*2)(29.2%)。不同IL-1基因多态性受试者的ABI差异无统计学意义(P>0.05)。HP阳性受试者ABI明显低于阴性者(P<0.01)。校正年龄、性别、高血压、糖尿病、抽烟状态、BMI、高脂血症及IL-1基因多态性后,ABI在HP阴性组及阳性组间差异无统计学意义(P>0.05),HP CagA抗体阳性是低ABI的危险因素(P<0.01)。结论:HP感染,尤其是CagA抗体阳性HP感染,可能是中国南方汉族中年人群动脉粥样硬化的一个危险因素,与2种IL-1基因位点多态性无明显相关性。

Objective:To investigate the effects of the gene polymorphism of IL-1-511 or IL-1RN VNTR on atherosclerosis in Helicobacter pylori(HP)infection aggravation healthy middle-aged people.Methods:The genotypes loci polymorphism of IL-1511 or IL-1RN VNTR in 227 healthy middle-aged people were determined using polymerase chain reaction(PCR)-restriction digestion analysis.The HP IgG,and CagA and VacA antibody were detected using ELISA,and the degree of atherosclerosis was assessed using the ankle-brachial index(ABI).Results:The main polymorphism of IL-1β-511 were CT(64.8%)and TT(22.3%),respectively.The allele frequency of four-repeat(IL-1RN*1)was 68.7%,and two repeat(IL-1RN*2)alleles was 29.2%in IL-1RN VNTR.The difference of ABI in subjects with different Il-1 gene polymorphisms was not statistically significant(P>0.05).The ABI in positive HP subjects was significantly lower than that in negative HP subjects(P<0.01).After adjusting the age,sex,hypertension,diabetes,smoking status,BMI,triglyceride and interleukin-1 Gene polymorphism,the difference of the ABI between positive and negative HP subjects was not statistically significant(P>0.05),and the positive HP CagA antibody was a low risk factor of ABI(P<0.01).Conclusions:The HP infection,especially CagA antibody-positive HP infection,may be a risk factor of atherosclerosis in the middle-aged Han population in southern China,and there is no significant correlation between it and polymorphisms of two IL-1 gene sites.