转录组测序及其在植物病原菌、植物与真菌互作研究中的应用与进展

Application and progresses in transcriptome sequencing for the study of plant pathogens and plant-fungal interactions

转录组测序(RNA sequencing,RNA-seq)技术是用来分析动态转录组的重要工具。RNA-seq生成的数据不仅可以量化基因表达,还有助于发现新的转录本、识别选择性剪接基因和检测等位基因特异性表达。本文主要综述了RNA-seq的技术步骤及工作原理,并介绍了其在植物病原菌及植物与真菌互作研究中的应用与进展。

Transcriptome sequencing(RNA-seq)is an important tool for analyzing dynamic transcriptomes.In addition to quantifying gene expression,RNA-seq generated data helping discover new transcripts,recognize alternative splicing genes and detect allele-specific expression.This paper mainly reviewed the technical steps and working principles of RNA-seq and introduced its application and progresses in the study of plant pathogens and plant-fungal interactions.