丁酸钠与丝兰对大肠杆菌K99攻毒哺乳犊牛生长性能及血清抗氧化指标的影响

Effects of Sodium Butyrate and Yucca on Growth Performance and Serum Antioxidant Indices of Sucking Calves Challenged with Escherichia coli K99

本试验旨在研究丁酸钠与丝兰对大肠杆菌K99攻毒哺乳犊牛生长性能及血清抗氧化指标的影响。试验选用40头健康、体重[(40±5)kg]相近的1日龄荷斯坦公犊牛,随机分为4组,每组10头。对照组(C组)饲喂基础饲粮,丁酸钠组(SB组)饲喂基础饲粮+30 g/d丁酸钠;丝兰组(Y组)饲喂基础饲粮+9 g/d丝兰,丁酸钠+丝兰组(SB+Y组)饲喂基础饲粮+30 g/d丁酸钠+9 g/d丝兰。在犊牛7日龄时进行大肠杆菌K99攻毒,试验进行至14日龄结束。结果表明:1)1~7日龄时,与SB组相比,SB+Y组犊牛平均日采食量显著降低(P<0.05)。2)11日龄时,SB组犊牛血清中过氧化氢酶(CAT)活性显著高于C组(P<0.05);14日龄时,SB组和Y组犊牛血清中CAT活性显著高于C组和SB+Y组(P<0.05)。11日龄时,与C组相比,Y组犊牛血清中谷胱甘肽过氧化物酶(GSH-Px)活性有升高的趋势(P=0.088),SB+Y组犊牛血清中GSH-Px活性显著提高(P<0.05)。7日龄时,与C组相比,Y组犊牛血清中丙二醛(MDA)含量有降低的趋势(P=0.050);14日龄时,与C组相比,SB+Y组犊牛血清中MDA含量有降低的趋势(P=0.089)。综上所述,哺乳犊牛处于大肠杆菌K99攻毒的应激模式下,丁酸钠与丝兰单独添加均可提高犊牛的抗氧化能力,但对犊牛的生长性能没有影响。丁酸钠与丝兰组合添加可提高犊牛的抗氧化能力,但降低了犊牛的平均日采食量。

This experiment was conducted to evaluate effects of sodium butyrate and Yucca on growth performance and serum antioxidant indices of sucking calves challenged with Escherichia coli K99.A total of 40 healthy Holstein calves with similar body weight of(40±5)kg were randomly allocated to 4 groups with 10 calves per group.The control group(C group)was fed a basic diet,the sodium butyrate group(SB group)was fed the basic diet+30 g/d sodium butyrate,the Yucca group(Y group)was fed the basic diet+9 g/d Yucca,and the sodium butyrate+Yucca group(SB+Y group)was fed the basic diet+30 g/d sodium butyrate+9 g/d Yucca.The calves were challenged with Escherichia coli K99 at 7 days of age,and the experiment ended at 14 days of age.The results showed as follows:1)during 1 to 7 days of age,compared with the SB group,the average daily feed intake of calves of SB+Y group was significantly decreased(P<0.05).2)At 11 days of age,the serum catalase(CAT)activity of calves of SB group was significantly higher than that of C group(P<0.05);at 14 days of age,the serum CAT activity of calves of SB group and Y group was significantly higher than that of C group and SB+Y group(P<0.05).At 11 days of age,compared with C group,the serum glutathione peroxidase(GSH-Px)activity of calves of Y group had a tendency to increase(P=0.088),and the serum GSH-Px activity of calves of SB+Y group was significantly increased(P<0.05).At 7 days of age,compared with C group,the serum malondialdehyde(MDA)content of calves of Y group had a tendency to decrease(P=0.050);at 7 days of age,compared with C group,the serum MDA content of calves of SB+Y group had a tendency to decrease(P=0.089).In conclusion,when sucking calves are under stress condition challenged with Escherichia coli K99,the separately supplementation of sodium butyrate and Yucca can improve the antioxidant capacity of calves,but it has no effect on the growth performance of calves.The combined supplementation of sodium butyrate and Yucca can improve the antioxidant capacity of calves,but reduce the average daily feed intake of calves.