蛋氨酸和生物素对体外培养绒山羊次级毛囊生长的影响

Effects of Methionine and Biotin on Growth of Secondary Follicles in Cashmere Goat

本研究旨在探讨蛋氨酸和生物素对绒山羊次级毛囊体外生长和形态的影响及作用。采用酶法和体外机械分离技术分离绒山羊次级毛囊,挑选出形态完整的次级毛囊用于毛囊体外培养。试验1:采用单因素试验设计,将分离出来的完整毛囊随机分为5组,每组4个重复,每个重复12根毛囊,共240根次级毛囊,对照组为Williams E基础培养液,试验组在基础培养液中依次添加7.5、15.0、30.0和60.0 mg/L蛋氨酸。试验2:采用单因素试验设计,将分离出来的完整毛囊随机分为4组,每组3个重复,每个重复10根毛囊,共120根次级毛囊,对照组为Williams E基础培养液,试验组在基础培养液中依次添加0.25、0.50和1.00 mg/L生物素。2个试验的毛囊均培养7 d,培养期间每隔24 h测量毛囊生长长度,并拍照测定其形态,试验结束后测定毛囊DNA合成量。试验1结果表明:与对照组比,15.0 mg/L蛋氨酸组次级毛囊累计长度和生长速度显著提高(P<0.05),毛球部形态保持最好。且15.0 mg/L蛋氨酸组的次级毛囊DNA含量最高。试验2结果表明:与对照组相比,0.50 mg/L生物素组次级毛囊累计长度和生长速度显著提高(P<0.05),且0.50 mg/L生物素组的次级毛囊DNA含量最高。由此可见,培养基中添加15.0 mg/L蛋氨酸或0.50 mg/L生物素能显著提高毛囊生长长度以及生长速度;且次级毛囊DNA含量最高。

The purpose of this study was to investigate the effects and functions of methionine and biotin on the growth and morphology of secondary hair follicles in cashmere goats.The secondary follicles of cashmere goats were separated by enzymatic method and in vitro mechanical separation technology,and secondary hair follicles with complete shape were selected for in vitro culture of hair follicles.Experiment 1:isolated intact hair follicles were randomly divided into 5 groups using a single factor test design,each with 4 repetitions,each with 12 hair follicles,a total of 240 secondary hair follicles,the control group was Williams E medium,and the test group was added with 7.5,15.0,30.0 and 60.0 mg/L methionine.Experiment 2:isolated intact hair follicles were randomly divided into 4 groups using a single factor test design,each with 3 repetitions,each with 10 hair follicles,a total of 120 secondary hair follicles,the control group was Williams E medium,and the test group was added with 0.25,0.50,1.00 mg/L biotin.All were cultured for 7 days,and the hair follicle growth length was measured every 24 h during the culture period,and photos were taken to determine the amount of hair follicle and DNA synthesis after the test.The results of experiment 1 showed as follows:compared with the control group,the 15 mg/L methionine significantly increased the secondary hair follicle growth length and growth rate(P<0.05),and the shape of the hair bulb remained the most.The DNA content in the 0.5 mg/L biotin group was the highest.The results of experiment 2 showed as follows:compared with the control group,the 0.50 mg/L biotin significantly increased the secondary hair follicle growth length and growth rate(P<0.05),and the secondary hair follicle DNA content in the 0.50 mg/L biotin group was the highest.From this,it can be seen that adding 15.0 mg/L methionine and 0.50 mg/L biotin can significantly increase the growth length and growth rate of hair follicles;and the secondary hair follicle DNA content of these two groups is the highest.