清血解毒合剂的高效液相色谱指纹图谱研究

Study on HPLC Fingerprint of Qingxue Jiedu Mixture

目的建立清血解毒合剂的高效液相色谱指纹图谱分析方法,为其科学的评价和质量控制提供实验依据。方法通过高效液相色谱方法,采用伊利特C 18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.05%磷酸溶液,梯度洗脱,柱温30℃,检测波长230 nm,流速1.0 mL·min^-1,进样量10μL,分析时间90 min,并采用国家药典委员会“中药色谱指纹图谱相似度评价系统”(2010版)软件对10批样品进行图谱处理。结果以26号峰黄芩苷为参照物,共标出36个共有峰,色谱峰分离度良好,并指认7个色谱峰,分别为没食子酸、原儿茶醛、儿茶素、咖啡酸、芍药苷、甘草苷和黄芩苷。结论该实验建立的指纹图谱方法准确,简单,稳定易操作,且专属性强,可作为清血解毒合剂质量控制的有效方法。

Objective To establish a high performance liquid chromatographic fingerprint analysis method for Qingxue jiedu mixture to provide experimental basis for its scientific evaluation and quality control.Methods High performance liquid chromatography was performed on an Elite C 18 column(4.6 mm×250 mm,5μm),and the mobile phase was acetonitrile-0.05%phosphoric acid for the gradient elution with column temperature of 30℃.The detection wavelength was set at 230 nm,and flow rate was 1.0 mL·min^-1.Injection volume was 10μL and the analysis time was 90 minutes.“Chinese medicine chromatographic fingerprint similarity evaluation system”(2010 version)software issued by the National Pharmacopoeia Commission was used for the processing of 10 batches of samples.Results With baicalin’s peak 26 as a reference,a total of 36 common peaks were identified with good resolution.The chromatographic peaks were well separated,and seven chromatographic peaks were identified as gallic acid,protocatechuic aldehyde,catechin,caffeic acid,paeoniflorin,glycyrrhizin,and baicalin.Conclusion The fingerprint method established in this experiment was accurate,simple,stable,and easy to operate,and had strong specificity.It can be used as an effective method for quality control of Qingxue Jiedu mixture.