含奎宁水凝胶的构建及其抗炎作用

Preparation of Hydrogel with Quinine and Its Anti-Inflammatory Effects

为提高透明质酸水凝胶的力学性能和释放药物的效果,通过引入海藻酸钠制作了透明质酸/海藻酸钠凝胶,并在其中加入了盐酸奎宁,对水凝胶的微观结构、理化性质和生物学性能进行了评价。红外光谱分析表明透明质酸、海藻酸钠、奎宁进行了良好的混合。SEM照片显示材料表面有均一的结构,内部有空洞。材料的力学性能和内部结构稳定。巨噬细胞细胞存活率实验表明水凝胶浸提液没有明显毒性,且可抑制脂多糖诱导的巨噬细胞活化,减少其释放炎症因子和细胞因子等炎症介质,获得的含奎宁水凝胶为皮肤材料提供了新的选择。

The biomedical applications of hyaluronic acid(HA)/sodium alginate(Alg)based hydrogels have been widely studied,showing the potentials in tissue engineering and wound healing.In this study,the quinine was introduced into the HA/Alg-based hydrogel to prepare anti-inflammatory hydrogel.Micro-structure,physicochemical properties were investigated.The results showed that the hydrogels possessed a uniform structure with pores inside,exhibited similar mechanical performance with or without quinine.Employing the in vitro model of lipopolysaccharides(LPS)-induced inflammation,the effects of the hydrogels on inflammation in cultured RAW 264.7 mouse macrophages was determined.The results showed that all the hydrogels did not affect the cell availability.In addition,the LPS-induced interleukin-1(IL-1),IL-6,tumor necrosis factor-αproduction was inhibited by the conditional medium of the hydrogels with quinine at 20-80 mg/mL,indicating its significant anti-inflammatory activity.Taken together,the prepared hydrogels with quinine may be an anti-inflammatory biomaterials and have potential application in skin wound healing.