芫参颗粒对正常小鼠抗氧化和抗缺氧作用的研究

Study on the Antioxidation and Anti-hypoxia Effects of Yuanshen Granule on Normal Mice

目的:研究芫参颗粒对正常小鼠的抗氧化和抗缺氧作用。方法:将800只小鼠随机分为空白对照组,芫参颗粒低、中、高剂量组(10、20、40 g/kg)和红景天口服液组(阳性对照,2 mL/kg),每组160只。各给药组小鼠灌胃相应药物,每天给药1次,连续给药30 d;空白对照组小鼠同法灌胃等容生理盐水。分别在给药后第1、3、7、14、30天各时间点,各组均随机选取8只小鼠分别进行常压、减压耐缺氧实验,心肌、脑组织中丙二醛(MDA)和超氧化物歧化酶(SOD)含量测定,以及H_2O_2诱导的红细胞溶血情况考察。结果:与空白对照组比较,芫参颗粒各剂量组小鼠的常压耐缺氧存活时间和减压耐缺氧存活时间(自给药7 d后)均显著延长(P<0.05);心肌、脑组织中MDA含量(分别自给药3或7 d后和自给药3、7或14 d后)均显著降低(P<0.05),SOD含量(分别自给药7、14或30 d后和自给药7 d后)均显著升高(P<0.05);红细胞溶血率(自给药3或7 d后)显著降低(P<0.05)。芫参颗粒中、高剂量组上述指标在部分时间点的改善效果显著优于红景天口服液组(P<0.05)。结论:芫参颗粒可有效延长正常小鼠的耐缺氧存活时间,对正常小鼠有抗氧化和抗缺氧的作用,且其中、高剂量的效果优于红景天口服液。

OBJECTIVE:To study the anti-oxidation and anti-hypoxia effect of Yuanshen granule on normal mice.METHODS:Totally 800 mice were randomly divided into blank control group,Yuanshen granule low-dose,middle-dose and high-dose groups(10,20,40 g/kg)and Hongjingtian oral liquid group(positive control,2 mL/kg),with 160 mice in each group.Administration groups were given relevant medicine intragastrically,once a day,for consecutive 30 d.Blank control group was given constant volume of normal saline by the same method.At 1st,3rd,7th,14th and 30th day after administration,8 mice in each group at each time point were randomly selected for anoxia tolerance test under normobaric pressure and decompression;the contents of MDA and SOD in myocardium and cerebral tissue were measured;the hemolysis effect of red blood cell induced by H2O2 was investigated.RESULTS:Compared with blank control group,the survival time of anoxia tolerance under normobaric pressure and under decompression(after 7 days of administration)were prolonged significantly in Yuanshen granule groups(P<0.05).The contents of MDA in myocardium and cerebral tissue(after 3 or 7 days of administration;after 3,7 or 14 days of administration)were significantly decreased(P<0.05),and the contents of SOD(after 7,14 or 30 days of administration;after 7 days of administration)were increased significantly(P<0.05);the erythrocyte hemolysis rate(after 3 or 7 days of administration)was significantly decreased(P<0.05).At some time points,the improvement effect of the above indexes in the Yuanshen granule middle-dose and high-dose groups was significantly better than that of Hongjingtian oral liquid group(P<0.05).CONCLUSIONS:Yuanshen granule can effectively prolong the hypoxia tolerance survival time of normal mice,it has anti-oxidant and anti-hypoxia effects on normal mice,and the effect of Yuanshen granule middle-dose and high-dose groups is better than that of Hongjingtian oral liquid group.