摘要
Phytoremediation is a sustainable remedial approach for removing benzene from environment.Plant associated bacteria could ameliorate the phytotoxic effects of benzene on plant,although the specificity of these interactions is unclear.Here,we used proteomics approach to gain a better understanding of the mechanisms involved in plant-bacteria interactions.Plant associated bacteria was isolated and subsequently inoculated into the sterilized Helianthus annuus,and the uptake rates of benzene by these inoculated plants were evaluated.At the end of the experiment,leaves and roots proteins were analyzed.The results showed inoculated H.annuus with strain EnL3 removed more benzene than other treatments after 96 h.EnL3 was identified as Enterobacter sp.according to 16S rDNA analysis.Based on the comparison of proteins,62 proteins were significantly up or down regulated in inoculated leaves,while 35 proteins were significantly up or down regulated in inoculated roots.Furthermore,there were 4 and 3 identified proteins presented only in inoculated H.annuus leaves and roots,respectively.These proteins involved in several functions including transcription and translation,photosynthesis,and stress response.The network among anti-oxidant defense system,protein synthesis,and photosynthetic electron transfer are involved in collaboratively activate the benzene uptake and stress tolerance in plant.
基金
This work was supported by the King Mongkut’s University of Technology Thonburi through the Post-doctoral Fellowship and the KMUTT 55th Anniversary Commemorative Fund.We thank Ms.Suthathip Kittisenachai,Mr.Yodying Yingchutrakul,and Mr.Sucheewin Krobthong of the National Center for Genetic Engineering and Biotechnology(BIOTEC)for their valuable help in the Proteomics Research Laboratory.
