27种丛枝菌根真菌接种对盆栽云木香生理生化及主要萜类成分的影响

Effects of 27 Strains of Arbuscular Mycorrhizal Fungi Inoculation on Physiology and Biochemistry and Major Components of Terpenoids in Potted Saussurea costus

在室温盆栽实验条件下,研究了27种外源性丛枝菌根(arbuscular mycorrhiza,AM)真菌对云木香幼苗的根系菌根侵染率、生理生化特性、根生物量及萜类成分积累的影响。研究结果表明,云木香与AM真菌形成了良好的共生关系,但27种AM真菌菌株与云木香二者之间存在共生偏好性。与对照组(CK)相比,部分AM真菌提高云木香叶片中可溶性蛋白和可溶性糖含量及抗氧化酶活性,降低丙二醛含量,增强云木香抗不利环境的能力;部分AM真菌处理组云木香根生物量显著增加(P<0.05);大部分AM真菌处理组云木香根中木香烃内酯、去氢木香内酯及总内酯含量显著增加(P<0.05),也有部分AM真菌处理组差异不明显或降低。从综合接种效应来看,易误巨孢囊霉(Gigaspora decipiens,Gd)、美丽盾巨孢囊霉(Scutellospora calospora,Sca)、瑚状盾巨囊霉(Racocetra coralloidea,Rco)、沙荒球囊霉(Septoglomus deserticola,Sde)、哥伦比亚内养囊霉(Entrophospora colombiana,Ec)、巴西类球囊霉(Paraglomus brasilianum,Pb)等是人工栽培条件下接种AM真菌云木香幼苗的潜在优良菌株,可为提高云木香产量和品质开辟一条新的途径。

A pot experiment was conducted to investigate the effects of 27 exogenous arbuscular mycorrhizal(AM)fungi strains on the root mycorrhiza infection rate,physiological and biochemical characteristics,root biomass and terpenoids accumulation of Aucklandia lappa seedlings at room temperature.A.lappa had a good symbiotic relationship with AM fungi,but there was difference in the preference of 27 exogenous AM fungi species for A.lappa.Compared with CK,some AM fungi increased the soluble protein and soluble sugar content and antioxidant enzyme activity in the leaves of A.lappa,reduced the malondialdehyde content,enhanced the ability of A.lappa to resist adverse environment.Biomass of the roots of A.lappa increased significantly in some AM fungal treatment groups.In most AM fungi treatment groups,the content of costunolide,dehydrocostus lactone and total lactones in the roots of A.lappa increased significantly(P<0.05),while some AM fungi treatment groups showed no significant difference or decrease.According to the comprehensive inoculation effect,Gigaspora decipiens,Scutellospora calospora,Racocetra coralloidea,Septoglomus deserticola,Entrophospora colombiana,Paraglomus brasilianum are the potential strains to inoculate A.lappa seedlings under artificial cultivation conditions,which could help to improve the yield and quality of A.lappa.