丛枝菌根真菌对黄花蒿生长和根系分泌物化学组成的影响

Effects of Arbuscular Mycorrhizal Fungi on Artemisia annua L.Growth and Chemical Composition of Root Exudates

研究盆栽条件下,接种与未接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)摩西球囊霉(Glomus mosseae)对黄花蒿(Artemisia annua L.)生长和根系分泌物化学组成的影响。结果表明,接种AMF黄花蒿的株高、苗干重和根干重均显著增加,增幅均达到20%以上;接种AMF也改善了黄花蒿的根系形态,除了根系半径以外,根长增加了87.0%、根表面积增加了97.0%、根体积增加了10.7%、根尖数增加了38.4%、根分叉数增加了75.6%、根系活力提高了19.6%,差异均达到极显著水平;与未接种AMF的黄花蒿基质中的根系分泌物(NM-S)相比,接种AMF下黄花蒿基质中的根系分泌物(AM-S)可溶性蛋白含量增加了74.38%,可溶性糖含量增加了16.13%,游离氨基酸含量增加了203%,有机酸的种类增多且含量显著提高;但接种AMF对黄花蒿水培液中根系分泌物含量的影响却呈现出了相反的作用。说明接种AMF有助于改善黄花蒿的根系形态,提高根系活力,在基质中促进根系分泌物的分泌,从而使黄花蒿汲取更多养分、提高生物量。

The effects of arbuscular mycorrhizal fungi(AMF)Glomus mosseae on the growth index and the root exudates chemical composition of Artemisia annua L.were studied under potted conditions.The results showed that the height,shoot dry weight and root dry weight of A.annua inoculated with AMF increased significantly,with an increase of more than 20%;in addition to root radius,root length,root surface area,root volume,tips,forks and root activity of A.annua increased by 87.0%,97.0%,10.7%,38.4%,75.6%and 19.6%,respectively;compared with the root exudates obtained from the matrix of A.annua without AMF inoculation(NM-S),the content of soluble protein,soluble sugar and free amino acid in the root exudates obtained from matrix of A.annua with AMF inoculation(AM-S)increased by 74.38%,16.13%and 203%,respectively,following the types and contents of organic acids increased significantly;but the effects of AMF on the root exudates of hydroponic solution showed an opposite trend.In the substrate,AMF may improve root morphology,root activity and secretion of the root exudates so that A.annua could absorb more nutrients and improve its biomass.